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Mouse anti Bovine MHC Class II DR antibody, clone CC108 (MCA5656GA) used for the evaluation of MHC class II expression on bovine myeloid cell sub-populations by flow cytometry.
Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and ?and 2C.2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14-CD16++ (red), CD14+CD16+ (blue) and CD14+CD16low/- (orange) compared to PBMC (black). Data shown are for one representative animal of four animals.
From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y,Entrican G, Glass EJ.
Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneicT-cell stimulatory capacity.
Vet Res. 2015 Sep 25;46:112.