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Mouse anti Bovine MHC class II DR antibody, clone CC108 (MCA5656) used for the evaluation of MHC class II expression on peripheral blood cells by flow cytometry.
CD8α+ SIRPα+ lung-resident DCs express cytokines that induce γ&delta T cells with suppressive phenotype. Phenotypic analysis of bovine lung DC subsets includes FSChigh MHC class II (A), SIRPα and CD11c (B), and SIRPα and CD8α (C). Cells were fixed, permeabilized, and stained for intracellular TGF-β and IL-10. DCs were gated on SIRPα- CD8α- double negatives (D), SIRPα-+ single positives (E), and SIRPα+ CD8α+ double positive (F). Dot plots are representative of tissues from six different animals. (G–I) SIRPα+ CD8α+ lung DCs are capable of inducing γδ T cells with suppressive phenotype. (G) Subpopulations of lung-resident DCs were FACS sorted and cocultured with autologous MACS-sorted γ&delta TCR+ T cells for 5 d. Proliferation was analyzed by CFSE dilution, and cells were stained for intracellular IL-10. (H) FMDV-specific proliferation of CD4+ T cells in the absence or presence of in vitro–expanded γδ T cells by SIRPα+ CD8α+ lung DCs and in Transwell plates with or without blocking anti–IL-10. (I) FMDV-specific IFN-? responses in CD4+ T cells in the presence or absence of in vitro–expanded autologous γ&delta T cells SIRP&alpha+ CD8+ lung DCs and in Transwell plates with or without blocking anti–IL-10. Bars indicate means of cells from four different animals analyzed in triplicate, and error bars indicate SEMs.
From: Guzman E, Hope J, Taylor G, Smith AL, Cubillos-Zapata C, Charleston B.
Bovine γδ T cells are a major regulatory T cell subset.
J Immunol. 2014 Jul 1;193(1):208-22.