MHC Class II DR Antibody | CC108

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MHC Class II DR Antibody | CC108 gallery image 1

Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR (MCA5656) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

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MHC Class II DR Antibody | CC108 gallery image 2

Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:FITC(MCA5656F)

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MHC Class II DR Antibody | CC108 gallery image 3

Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:RPE(MCA5656PE)

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MHC Class II DR Antibody | CC108 gallery image 4

Published customer image:
Mouse anti Bovine MHC Class II DR antibody, clone CC108 used for the evaluation of MHC class II expression on bovine myeloid cell sub-populations.
Image caption:
Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and ?and 2C.2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14-CD16++ (red), CD14+CD16+ (blue) and CD14+CD16low/- (orange) compared to PBMC (black). Data shown are for one representative animal of four animals.

From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y,Entrican G, Glass EJ.
Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneicT-cell stimulatory capacity.
Vet Res. 2015 Sep 25;46:112.

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MHC Class II DR Antibody | CC108 gallery image 5

Published customer image:
Mouse anti Bovine MHC class II DR antibody, clone CC108 (MCA5656) used for the evaluation of MHC class II expression on peripheral blood cells by flow cytometry.
Image caption:
Monocyte/macrophages and MoDCs express IL-10 and TGF-β, and induce proliferation of IL-10+ γδ T cells. Peripheral blood monocyte/macrophages expressing CD14 (A) and expression of MHC class II/CD1b (B) in CD14+ cells. After a 3-d culture in the presence of GM-CSF and IL-4, the cells increased expression of MHC class II and CD1b (C). IL-10 and TGF-β expression in ex vivo CD14+ cells (D) and cultured MoDCs (E). Both monocyte/macrophages and MoDCs induced the expansion of autologous IL-10–expressing γδ T cells (F). Bar graph shows means (n = 10) and error bars indicate SEMs. Representative plots of cells obtained from 10 different animals analyzed in duplicate. Quadrants were placed based on isotype and fluorochrome controls.

From: Guzman E, Hope J, Taylor G, Smith AL, Cubillos-Zapata C, Charleston B.
Bovine γδ T cells are a major regulatory T cell subset.
J Immunol. 2014 Jul 1;193(1):208-22.

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MHC Class II DR Antibody | CC108 gallery image 6

Published customer image:
Mouse anti Bovine MHC class II DR antibody, clone CC108 (MCA5656) used for the evaluation of MHC class II expression on peripheral blood cells by flow cytometry.
Image caption:
CD8α+ SIRPα+ lung-resident DCs express cytokines that induce γ&delta T cells with suppressive phenotype. Phenotypic analysis of bovine lung DC subsets includes FSChigh MHC class II (A), SIRPα and CD11c (B), and SIRPα and CD8α (C). Cells were fixed, permeabilized, and stained for intracellular TGF-β and IL-10. DCs were gated on SIRPα- CD8α- double negatives (D), SIRPα-+ single positives (E), and SIRPα+ CD8α+ double positive (F). Dot plots are representative of tissues from six different animals. (G–I) SIRPα+ CD8α+ lung DCs are capable of inducing γδ T cells with suppressive phenotype. (G) Subpopulations of lung-resident DCs were FACS sorted and cocultured with autologous MACS-sorted γ&delta TCR+ T cells for 5 d. Proliferation was analyzed by CFSE dilution, and cells were stained for intracellular IL-10. (H) FMDV-specific proliferation of CD4+ T cells in the absence or presence of in vitro–expanded γδ T cells by SIRPα+ CD8α+ lung DCs and in Transwell plates with or without blocking anti–IL-10. (I) FMDV-specific IFN-? responses in CD4+ T cells in the presence or absence of in vitro–expanded autologous γ&delta T cells SIRP&alpha+ CD8+ lung DCs and in Transwell plates with or without blocking anti–IL-10. Bars indicate means of cells from four different animals analyzed in triplicate, and error bars indicate SEMs.

From: Guzman E, Hope J, Taylor G, Smith AL, Cubillos-Zapata C, Charleston B.
Bovine γδ T cells are a major regulatory T cell subset.
J Immunol. 2014 Jul 1;193(1):208-22.

Enlarge
MHC Class II DR Antibody | CC108 gallery image 7

Published customer image:
Mouse anti Bovine MHC class II DR antibody, clone CC108 (MCA5656) used for the evaluation of MHC class II expression on peripheral blood cells by flow cytometry.
Image caption:
CD8a- SIRPa- ALDCs express cytokines that induce ?d T with a regulatory phenotype. Phenotypic analysis of bovine ALDC subsets includes FSChigh MHC class II (A), SIRPa and CD11c (B), and SIRPa and CD8a (C). Cells were fixed, permeabilized, and stained for intracellular TGF-ß and IL-10. DCs were gated on SIRPa+ CD8a- (D), SIRPa+ CD8a+ (E), and SIRPa- CD8a- double negative (F). Dot plots are representative of cells from five different animals. (G–I) SIRPa- CD8a- ALDCs are capable of inducing ?d T cells with suppressive phenotype. (G) Subpopulations of ALDCs were FACS sorted and cocultured with autologous MACS-sorted ?d TCR+ T cells for 5 d. Proliferation was measured by CFSE dilution and intracellular staining of IL-10 by flow cytometry. (H) FMDV-specific proliferation of CD4+ T cells in the absence or presence of in vitro–expanded autologous ?d T cells by SIRPa- CD8a- ALDCs and in Transwell plates with or without blocking anti–IL-10. (I) FMDV-specific IFN-? responses in CD4+ T cells in the presence or absence of in vitro–expanded autologous ?d T cells SIRPa- CD8a- ALDCs and in Transwell plates with or without blocking anti–IL-10. Bars indicate means of cells from four different animals analyzed in triplicate, and error bars indicate SEMs.

From: Guzman E, Hope J, Taylor G, Smith AL, Cubillos-Zapata C, Charleston B.
Bovine γδ T cells are a major regulatory T cell subset.
J Immunol. 2014 Jul 1;193(1):208-22.

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  • Mouse anti Bovine MHC Class II DR
  • Mouse anti Bovine MHC Class II DR:FITC
  • Mouse anti Bovine MHC Class II DR:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC108
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5656FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5656F
    MCA5656Fdatasheet pdfdatasheet pdf0.25 mg
    MCA5656
    MCA5656PEFdatasheet pdfdatasheet pdf100 Tests
    MCA5656PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Bovine MHC class II DR antibody, clone CC108 recognizes Bovine MHC Class II DR. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

      The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1.0ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the Mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional MHC Class II DR Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class II DR Antibody : Purified CC108 F 0.25 mg
    MHC Class II DR Antibody : RPE CC108 F 100 Tests
    MHC Class II DR Antibody : FITC CC108 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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