IL-2 Antibody | AbD21766

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Bovine Interleukin-2 ELISA

Recombinant bovine interleukin-2 detected using Human anti Bovine interleukin-2, clone AbD21766 as the capture antibody and Human anti Bovine interleukin-2 clone AbD22297 (HCA268) followed by Mouse anti Histidine Tag:HRP (MCA1396P) as the detection reagent

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Bovine Interleukin-2 Intracellular Flow Cytometry Staining of Native Bovine Interleukin-2 (IL-2)

PBMC from naturally Mycobacterium bovis infected cattle were stained using Human anti Bovine interleukin-2 antibody, clone AbD21766, conjugated with Alexa Fluor 647 (HCA267A467) at a dilution of 1:8

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  • Human anti Bovine Interleukin-2:Alexa Fluor® 647
  • Human anti Bovine Interleukin-2
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  • Product Type
    Monoclonal Antibody
  • Clone
    AbD21766
  • Isotype
    HuCAL Fab monovalent
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA267E, ES, Fdatasheet pdfdatasheet pdf0.1 mg
    HCA267
    HCA267A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    HCA267A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Bovine Interleukin-2, clone AbD21766 recognizes bovine interleukin-2 (IL-2), also known as T-cell growth factor (TCGF). Bovine IL-2 is a 155 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.

      Human anti bovine interleukin-2, clone AbD21766, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 using intracellular flow cytometry, ELISPOT and ELISA.

      In ELISA and ELISPOT clone AbD21766 functions as the capture antibody in a sensitive assay for bovine IL-2 determination.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to Alexa Fluor 647 (A647) - liquid.
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.01% Thiomersal
      1% Bovine Serum Albumin
      0.01% Thiomersal
    • Immunogen
      Fc-fusion protein containing the amino acid sequence 21-155 from bovine interleukin-2.
    • Purity
    • Affinity
      The monovalent intrinsic affinity of this antibody was measured as KD=0.3 nM by real time, label-free molecular interaction analysis on recombinant bovine interleukin-2.
    • Approx. Protein Concentrations
      Antibody concentration 0.05 mg/ml
      Antibody concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
    • GO Terms
      cytokine activity
      growth factor activity
      extracellular space
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany. This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      ELISpot
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • ELISA
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with HCA268P as the detection reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-2 Antibody Formats

    Formats Clone Applications Sizes available
    IL-2 Antibody : Alexa Fluor® 647 AbD21766 F 100 Tests/1ml
    IL-2 Antibody : Purified AbD21766 E, ES, F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-FH Negative ControlHCA0450.1 mgC, E, P, WB
        HCA045

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Leucoperm™BUF0950 TestsF
          BUF09
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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