Rat IgG2a Negative Control antibody
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Rat IgG2a Negative Control:APC
- Product Type
- Negative/Isotype Control
- Rat IgG2a Negative Control
|Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.
Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) APC 650 661
- For research purposes only
- 12 months from date of despatch
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Rat IgG2a Negative Control antibody
Barraud-Lange, V. et al. (2020) Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.
Int J Mol Sci. 21 (22)Nov 11 [Epub ahead of print].
do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
Research in Veterinary Science. 145: 193-204.
Karayannopoulou, M. et al. (2022) Effect of major versus minor mastectomy on host immunity in canine mammary cancer
Vet Immunol Immunopathol. Feb 24: 110403.
Matralis, D.T. et al. (2023) Intracellular IFN-γ and IL-4 levels of CD4 + and CD8 + T cells in the peripheral blood of naturally infected (Leishmania infantum) symptomatic dogs before and following a 4-week treatment with miltefosine and allopurinol: a double-blinded, controlled and cross-sectional study.
Acta Vet Scand. 65 (1): 2.
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