Rat IgG2a Negative Control antibody
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F ResetRat IgG2a Negative Control:Alexa Fluor® 647
- Product Type
- Negative/Isotype Control
- Isotype
- IgG2a
- Specificity
- Rat IgG2a Negative Control
Antibody Quality and Performance Guarantee
| Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry. Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells. |
|
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Trinitrophenol
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
* |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Rat IgG2a Negative Control antibody
-
Barraud-Lange, V. et al. (2020) Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.
Int J Mol Sci. 21 (22)Nov 11 [Epub ahead of print]. -
do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
Research in Veterinary Science. 145: 193-204. -
Karayannopoulou, M. et al. (2022) Effect of major versus minor mastectomy on host immunity in canine mammary cancer
Vet Immunol Immunopathol. Feb 24: 110403. -
Matralis, D.T. et al. (2023) Intracellular IFN-γ and IL-4 levels of CD4 + and CD8 + T cells in the peripheral blood of naturally infected (Leishmania infantum) symptomatic dogs before and following a 4-week treatment with miltefosine and allopurinol: a double-blinded, controlled and cross-sectional study.
Acta Vet Scand. 65 (1): 2.
MCA6005A647
0215
166470
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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