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Rat IgG2a Negative Control antibody

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Rat IgG2a Negative Control Antibody (TNP) thumbnail image 1 Rat IgG2a Negative Control Antibody (TNP) thumbnail image 2 Rat IgG2a Negative Control Antibody (TNP) thumbnail image 3 Rat IgG2a Negative Control Antibody (TNP) thumbnail image 4
Rat IgG2a Negative Control Antibody (TNP) gallery image 1
Figure A. A647 conjugated Rat anti Canine CD4 (MCA1038A647) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A647 conjugated Rat anti Canine CD4 (MCA1038A647) and RPE conjugated Rat anti Canine CD5 (MCA1037PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Negative Control Antibody (TNP) gallery image 2
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Negative Control Antibody (TNP) gallery image 3
Figure A. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Rat anti Canine CD45R (MCA1043PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Negative Control Antibody (TNP) gallery image 4
Figure A. RPE conjugated Rat anti Canine CD45R (MCA1043PE) and A488 conjugated Rat IgG1 isotype control (MCA6005A488). Figure B. RPE conjugated Rat anti Canine CD45R (MCA1043PE) and A488 conjugated Rat anti Canine CD44 (MCA1041A488). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Rat IgG2a Negative Control:Alexa Fluor® 488

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:Alexa Fluor® 647

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:APC

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:Biotin

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:FITC

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:Low Endotoxin

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:PacificBlue®

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:RPE

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:RPE-Cy7

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Rat IgG2a Negative Control:Amethyst Orange

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).

Product Type
Negative/Isotype Control
Isotype
IgG2a
Isotype controls savings up to 65%
Product Code Applications Pack Size List Price Quantity
MCA6005A488
Datasheet
F
MSDS
100 Tests/1ml loader

MCA6005A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA6005APC
Datasheet
F
MSDS
100 Tests loader

MCA6005B
Datasheet
F
MSDS
0.1 mg loader

MCA6005F
Datasheet
F
MSDS
0.1 mg loader

MCA6005EL
Datasheet
F
MSDS
0.5 mg loader

MCA6005PB
Datasheet
F
MSDS
100 Tests/1ml loader

MCA6005GA
Datasheet
F
MSDS
0.1 mg loader

MCA6005
Datasheet
F
MSDS
1 mg loader

MCA6005PE
Datasheet
F
MSDS
100 Tests loader

MCA6005PECY7
Datasheet
F
MSDS
100 Tests/1ml loader

MCA6005AMO
Datasheet
F
MSDS
0.1 mg loader

Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.

Product Details

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE)-Cy7 - lyophilised
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 1.0 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Trinitrophenol
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4oC or at -20oC if preferred.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of reconstitution
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of reconstitution.
Guarantee
12 months from date of reconstitution
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
Cy® and Cydye® are registered trademarks of GE Healthcare
Regulatory
For research purposes only

Applications of Rat IgG2a Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry Neat
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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