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Rat IgG2a Negative Control antibody

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Rat IgG2a Negative Control Antibody (TNP) thumbnail image 1 Rat IgG2a Negative Control Antibody (TNP) thumbnail image 2 Rat IgG2a Negative Control Antibody (TNP) thumbnail image 3 Rat IgG2a Negative Control Antibody (TNP) thumbnail image 4
Rat IgG2a Negative Control Antibody (TNP) gallery image 1
Figure A. A647 conjugated Rat anti Canine CD4 (MCA1038A647) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A647 conjugated Rat anti Canine CD4 (MCA1038A647) and RPE conjugated Rat anti Canine CD5 (MCA1037PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Negative Control Antibody (TNP) gallery image 2
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Negative Control Antibody (TNP) gallery image 3
Figure A. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Rat anti Canine CD45R (MCA1043PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Negative Control Antibody (TNP) gallery image 4
Figure A. RPE conjugated Rat anti Canine CD45R (MCA1043PE) and A488 conjugated Rat IgG1 isotype control (MCA6005A488). Figure B. RPE conjugated Rat anti Canine CD45R (MCA1043PE) and A488 conjugated Rat anti Canine CD44 (MCA1041A488). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2a Negative Control:Alexa Fluor® 488

Product Type
Negative/Isotype Control
Isotype
IgG2a
Specificity
Rat IgG2a Negative Control

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MCA6005A488
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Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.		 Isotype controls savings up to 65%

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Trinitrophenol
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

References for Rat IgG2a Negative Control antibody

  1. Barraud-Lange, V. et al. (2020) Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.
    Int J Mol Sci. 21 (22)Nov 11 [Epub ahead of print].
  2. do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
    Research in Veterinary Science. 145: 193-204.
  3. Karayannopoulou, M. et al. (2022) Effect of major versus minor mastectomy on host immunity in canine mammary cancer
    Vet Immunol Immunopathol. Feb 24: 110403.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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