Rat IgG2a Negative Control antibody

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Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 1

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 2

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 3

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 4

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 5

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 1 — Figure A. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat anti mouse CD40 (MCA1143PE). All experiments performed on mouse splenocytes in the presence of mouse SeroBlock (BUF041A)

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 2 — Figure A. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat IgG2a isotype control (MCA1212A488). Figure B. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat anti Mouse CD45R (MCA1258A88). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 3 — Figure A. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat anti Mouse CD48 (MCA925PE). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 4 — Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat anti Canine CD4 (MCA1038PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 5 — Figure A. RPE conjugated Rat anti Canine CD5 (MCA1037PE) and A647 conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated Rat anti Canine CD5 (MCA1037PE) and A647 conjugated Mouse anti Canine CD4 (MCA1038A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Rat IgG2a Negative Control:Alexa Fluor® 405

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Alexa Fluor® 488

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Alexa Fluor® 647

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Preservative Free

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Biotin

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:FITC

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Low Endotoxin

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Pacific Blue®

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:RPE

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:RPE-Alexa Fluor® 647

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Rat IgG2a Negative Control:Alexa Fluor® 700

Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.

Product Type: Negative/Isotype Control
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA1212A405	F	100 Tests/1ml
MCA1212A488	F	100 Tests/1ml
MCA1212A647	F	100 Tests/1ml
MCA1212XZ	E F	1 mg
MCA1212B	F	0.1 mg
MCA1212F	F	100 Tests
MCA1212EL	F	0.5 mg
MCA1212PB	F	100 Tests/1ml
MCA1212	E F	1 ml
MCA1212PE	F	100 Tests
MCA1212P647	F	100 Tests/1ml
MCA1212A700	F	100 Tests/1ml

Rat IgG2a Negative Control antibody is suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells.

Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with dog cells.

N.B.This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.

Product Details

Target Species

Negative Control

Product Form

Purified IgG conjugated to Alexa Fluor® 405 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Pacific Blue® - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) Alexa Fluor® 647 - lyophilized

Product Form

Purified IgG conjugated to Alexa Fluor® 700 - liquid

Reconstitution

Reconstitute with 1 ml distilled water

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Carrier Free

Yes

Carrier Free

Yes

Immunogen

Human lymphocytes.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Fusion Partners

Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.
Guarantee: 12 months from date of reconstitution.
Guarantee: 18 months from date of despatch.

More Information

Acknowledgements: The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Acknowledgements: This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of Rat IgG2a Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		*
Flow Cytometry		*
Flow Cytometry		*
ELISA
Flow Cytometry		*
Immunohistology - Frozen
Flow Cytometry		*
Flow Cytometry		1/10*
Flow Cytometry		*
Flow Cytometry		*
ELISA
Flow Cytometry		*
Immunohistology - Frozen
Flow Cytometry		*
Flow Cytometry	*	*
Flow Cytometry		*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.* It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagents.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
* It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
* It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Application Based External Images

Flow Cytometry

Product Specific References

References for Rat IgG2a Negative Control antibody

Sumagin, R. et al. (2008) Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.
Am J Physiol Heart Circ Physiol. 295: H969-H977.
Chiu, W.C. et al. (2011) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
J Nutr Biochem. 20: 254-60.
Guilloteau, L.A. et al. (2003) Nramp1 is not a major determinant in the control of Brucella melitensis infection in mice.
Infect Immun. 71: 621-8.
Stapleton, T.W. et al. (2000) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications.
Tissue Eng Part A. 17: 231-42.
Park, S.W. et al. (2012) A1 adenosine receptor allosteric enhancer PD-81723 protects against renal ischemia-reperfusion injury.
Am J Physiol Renal Physiol. 303: F721-32.
Schmidt, E.P. et al. (2012) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
Nat Med. 18 (8): 1217-23.
McConnell, M.J. et al. (2009) H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning.
Proc Natl Acad Sci U S A. 106: 6784-9.
Rabadi MM et al. (2016) Peptidyl arginine deiminase-4 deficient mice are protected against kidney and liver injury after renal ischemia and reperfusion.
Am J Physiol Renal Physiol. Jun 22: ajprenal.00254.2016. [Epub ahead of print]
Rabadi, M.M. et al. (2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NFκB Essential Modulator).
Am J Physiol Renal Physiol. Apr 03 [Epub ahead of print].

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