Multiple IgG1/IgG2a/IgG1 Negative Control antibody

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Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve Control

Product Type
Negative/Isotype Control
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
TC023 F* 100 Tests/1ml
Multiple IgG1/IgG2a/IgG1 negative control is a suitable isotype control for the measurement of non-specific binding of mouse IgG1, rat IgG2a and rat IgG1 monoclonal antibodies, conjugated to FITC, RPE and Alexa Fluor ® 647 respectively, in three colour flow cytometry experiments.

This product is appropriate for use in experiments targeting human, canine and porcine cells.

Product Details

Target Species
Negative Control
Product Form
Triple Color combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT)
A647 reagent: IgG1 (RAT)

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Multiple IgG1/IgG2a/IgG1 Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1It is recomended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul