Mouse IgM Negative Control antibody

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Mouse IgM Negative Control:FITC

Mouse IgM isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Mouse IgM Negative Control

Mouse IgM isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.

Product Type
Negative/Isotype Control
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA692F F 0.1 mg
MCA692 F 100 Tests
Mouse IgM negative control antibody is designed for use as a negative control reagent to assess the level of non-specific binding of mouse IgM monoclonal antibodies to human cells.

This antibody recognizes a cell surface protein on rat cells and is therefore not suitable as an isotype control for work on this species.

Product Details

Target Species
Negative Control
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
Preparation
Purified IgM prepared by ammonium sulphate precipiation from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Mouse IgM Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

* We recommend using this reagent at the same concentration as the test reagent.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood.