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Mouse IgM Negative Control antibody

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Mouse IgM Negative Control Antibody thumbnail image 1 Mouse IgM Negative Control Antibody thumbnail image 2
Mouse IgM Negative Control Antibody gallery image 1
Figure A. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse IgM isotype control (MCA692) detected with goat anti mouse IgM PE (STAR86PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM (STAR86PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgM Negative Control Antibody gallery image 2
Figure A. RPE conjugated mouse anti rat CD2 (MCA154PE) and FITC conjugated mouse IgM isotype control (MCA692F). Figure B. RPE conjugated mouse anti rat CD2 (MCA154PE) and FITC conjugated mouse anti rat CD3 (MCA772F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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Mouse IgM Negative Control

Product Type
Negative/Isotype Control
Isotype
IgM
Specificity
Mouse IgM Negative Control

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Mouse IgM negative control antibody is designed for use as a negative control reagent to assess the level of non-specific binding of mouse IgM monoclonal antibodies to human cells.

This antibody recognizes a cell surface protein on rat cells and is therefore not suitable as an isotype control for work on this species.

Target Species
Negative Control
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipiation from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgM concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood.

References for Mouse IgM Negative Control antibody

  1. Peek, V. et al. (2020) Age-Dependent Changes of Adipokine and Cytokine Secretion From Rat Adipose Tissue by Endogenous and Exogenous Toll-Like Receptor Agonists.
    Front Immunol. 11: 1800.

RRID
AB_322278
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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