Mouse IgM Negative Control antibody
Mouse IgM Negative Control:FITC
Mouse IgM isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.
Mouse IgM Negative Control
Mouse IgM isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.
- Product Type
- Negative/Isotype Control
- Isotype
- IgM
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA692F | F | 0.1 mg |
|
|
| MCA692 | F | 100 Tests |
|
Mouse IgM negative control antibody is designed for use as a negative control reagent to assess the level of non-specific binding of mouse IgM monoclonal antibodies to human cells.
This antibody recognizes a cell surface protein on rat cells and is therefore not suitable as an isotype control for work on this species.
This antibody recognizes a cell surface protein on rat cells and is therefore not suitable as an isotype control for work on this species.
Product Details
- Target Species
- Negative Control
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
- Preparation
- Purified IgM prepared by ammonium sulphate precipiation from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgM concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Mouse IgM Negative Control antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | * | ||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
* We recommend using this reagent at the same concentration as the test reagent.
* We recommend using this reagent at the same concentration as the test reagent.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Mouse IgM Negative Control antibody
-
Peek, V. et al. (2020) Age-Dependent Changes of Adipokine and Cytokine Secretion From Rat Adipose Tissue by Endogenous and Exogenous Toll-Like Receptor Agonists.
Front Immunol. 11: 1800.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
