Mouse IgG2a Negative Control antibody
Mouse IgG2a Negative Control:RPE
- Product Type
- Negative/Isotype Control
- Isotype
- IgG2a
- Specificity
- Mouse IgG2a Negative Control
Antibody Quality and Performance Guarantee
| Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages. Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES. |
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Human T lymphocytes.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
* |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Mouse IgG2a Negative Control antibody
-
Fijak, M. et al. (2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
Am J Reprod Immunol. 74 (1): 12-25.
- RRID
- AB_322514
MCA1210PE
151526
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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