Mouse IgG2a Negative Control antibody
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F ResetMouse IgG2a Negative Control:FITC
- Product Type
- Negative/Isotype Control
- Isotype
- IgG2a
- Specificity
- Mouse IgG2a Negative Control
Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages. Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES. |
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Human T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | * |
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Mouse IgG2a Negative Control antibody
-
Fijak, M. et al. (2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
Am J Reprod Immunol. 74 (1): 12-25. -
Midavaine, É. et al. (2024) Discovery of a CCR2-targeting pepducin therapy for chronic pain.
Pharmacol Res. : 107242.
- RRID
- AB_567343
MCA1210F
162310 1806If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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