Mouse IgG1 Negative Control antibody
8.75 µl of each IP was loaded onto an AnykD Criterion TGX Stain-Free gel. 8.75 μl Jurkat whole cell lysate (WCL) was run in lanes 5 & 11 while Precision Plus Protein prestained standards were run in lane 6.
Rabbit anti GAPDH antibody (VPA00187) was used at 1/1000 in lanes 1-5. HRP conjugated Tidyblot (STAR209P) was used at 1/200 and visualized on the Bio-Rad Chemidoc Touch Imaging System. Arrow points to GAPDH (molecular weight 37 kDa).
RPE and FITC conjugated Rat IgG1 isotype control antibodies (MCA1209PE, MCA1209F) used to demonstrate background staining intensity on rat adrenal cells by flow cytometry.
Image caption:
Intraadrenal MHCII+/CD86+ cells during collagen type II induced arthritis (CIA).
Quantifcation of MHCII and CD86 positive adrenal gland cells (exemplary FACS staining).
From: Stangl H, Krammetsvogl A, Lesiak M, Wolff C, Straub RH.
MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.
Sci Rep. 2020 Oct 13;10(1):17071.
doi: 10.1038/s41598-020-74309-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F WB ResetMouse IgG1 Negative Control
- Product Type
- Negative/Isotype Control
- Isotype
- IgG1
- Specificity
- Mouse IgG1 Negative Control
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| Mouse IgG1 Negative Control antibody is suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages. Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES |
- Target Species
- Negative Control
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Human T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
* |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.* It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for Mouse IgG1 Negative Control antibody
-
Weiss, D.J. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
J Leukoc Biol. 83 (1): 48-55. -
Chen, W. et al. (2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.
Mol Vis. 15: 619-28. -
Safeukui I et al. (2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.
MBio. 6 (1) pii: e02493-14. -
Aricha, R. et al. (2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.
J Autoimmun. 67: 57-64. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Stangl, H. et al. (2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.
Sci Rep. 10 (1): 17071.
- RRID
- AB_322265
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