Recombinant chicken inerleukin-6 is a histidine tagged molecule produced in E. coli. Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells. It plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses.
Recombinant chicken inerleukin-6 induces corticosterone secretion in vivo and induces proliferation of the IL-6 dependant murine hybridoma cell line 7TD1 in vitro (Marais et al. 2011).
Recombinant chicken inerleukin-6 (PAP003) is the immunogen used to produce Bio-Rad chicken IL-6 antibody (AHP942Z).
Purified His-tagged recombinant protein expressed in E.coli - liquid
Recombinant protein prepared from E. coli.
>85% by Ni Chelate chromatography
Approx. Protein Concentrations
Protein Molecular Weight
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
In vitro Assay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.