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Recombinant Chicken interleukin-6 (PAP003) detected with horseradish peroxidase conjugated Mouse anti Chicken interleukin-6:HRP (MCA5932P)

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  • Recombinant Chicken Interleukin-6
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PAP003FN, IAdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant chicken inerleukin-6 is a histidine tagged molecule produced in E. coli. Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells. It plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses.

      Recombinant chicken inerleukin-6 induces corticosterone secretion in vivo and induces proliferation of the IL-6 dependant murine hybridoma cell line 7TD1 in vitro (Marais et al. 2011).

      Recombinant chicken inerleukin-6 (PAP003) is the immunogen used to produce Bio-Rad chicken IL-6 antibody (AHP942Z).
    • Intended Use
    • Target Species
    • Product Form
      Purified His-tagged recombinant protein expressed in E.coli - liquid
    • Reconstitution
    • Preparation
      Recombinant protein prepared from E. coli.
    • Preservative Stabilisers
      None present
    • Purity
      >85% by Ni Chelate chromatography
    • Approx. Protein Concentrations
      500 ug/ml
    • Protein Molecular Weight
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of despatch.
    • GO Terms
      cytokine activity
      extracellular space
      interleukin-6 receptor binding
      immune response
      acute-phase response
      growth factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      His-tag is a registered trademark of EMD Biosciences.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
      In vitro Assay

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-6 Formats

    Formats Applications Sizes available
    IL-6 : Rec. Protein FN, IA 50 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Further Reading

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