CD25 Antibody | 13504

//www.bio-rad-antibodies.com/chicken-cd25-antibody-13504-hca173.html
CD25 Antibody | 13504 gallery image 1

Chicken PBMCs (stimulated for 3 days with concanavalin A) stained with Human anti Chicken CD25 (HCA173)

Enlarge
CD25 Antibody | 13504 gallery image 2

Chicken PBMCs (stimulated for 3 days with concanavalin A) stained with Human anti Chicken CD25:FITC (HCA173F)

Enlarge
CD25 Antibody | 13504 gallery image 3

Chicken PBMCs (stimulated for 3 days with concanavalin A) stained with Human anti Chicken CD25:Alexa Fluor®647 (HCA173A647)

Enlarge
  • CD25 Antibody | 13504 thumbnail image 1
  • CD25 Antibody | 13504 thumbnail image 2
  • CD25 Antibody | 13504 thumbnail image 3
  • Human anti Chicken CD25:Alexa Fluor® 647
  • Human anti Chicken CD25:FITC
  • Human anti Chicken CD25
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    13504
  • Isotype
    HuCAL Fab bivalent
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA173FFdatasheet pdfdatasheet pdf0.1 mg
    HCA173F
    HCA173Fdatasheet pdfdatasheet pdf0.1 mg
    HCA173
    HCA173A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    HCA173A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Chicken CD25 antibody, clone 13504 recognizes the chicken IL-2 Receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature.
      Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
      CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
      Chicken IL-2Rα is the low affinity receptor for Interleukin-2.
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Duckno
      Gooseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to Alexa Fluor 647 (A647) - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinantly expressed his-tagged chicken CD25
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.05mg/ml
      Antibody concentration 0.1mg/ml
      Antibody concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
      Phosphate buffered saline.
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : Purified 13504 F 0.1 mg
    CD25 Antibody : FITC 13504 F 0.1 mg
    CD25 Antibody : Alexa Fluor® 647 13504 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti Histidine Tag:DyLight®549MCA1396D549GA0.1 mgF, IF
      MCA1396D549GA
      Mouse anti Histidine Tag:DyLight®800MCA1396D800GA0.1 mgWB
      MCA1396D800GA
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647HCA052A6471 mlE, F
        HCA052A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative Control:FITCHCA052F0.1 mgC, E, F, P, WB
        HCA052F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD4:FITCMCA2164F0.1 mgF
          MCA2164F
          Mouse anti Chicken CD8 Alpha:FITCMCA2166F0.1 mgF
          MCA2166F
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA
          Mouse anti Chicken CD8 Alpha:RPEMCA2166PE0.1 mgF
          MCA2166PE
          Mouse anti Chicken CD4:RPEMCA2164PE100 TestsF
          MCA2164PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA
          Mouse anti Chicken CD8 Alpha:RPEMCA2166PE0.1 mgF
          MCA2166PE
          Mouse anti Chicken CD4:RPEMCA2164PE100 TestsF
          MCA2164PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA
          Mouse anti Chicken CD4MCA21640.25 mgC, F, IP
          MCA2164

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...

              You May Also Be Interested In...