CD4 Antibody | vpg34

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CD4 Antibody | vpg34 gallery image 1

Published customer image:
FITC conjugated Mouse anti Feline CD4 antibody, clone vpg34 (MCA1346F) used for the identification of CD4+ lymphocytes in peripheral blood smears by immunocytochemistry.
Image caption:
Lymphocyte subsets after M. haemofelis exposure in the ten SPF cats. The five cats in group A had undergone previous “Cand. M. turicensis” infection and the five cats in group B were naïve control cats. The M. haemofelis exposure took place on day 0. A) T cells (CD5+?MHCII+) counts, B) CD4+ T cell counts, C) CD8+ T cell counts, D) CD4+/CD8+ ratio, E) CD4+CD25+ activated T cells counts and F) B cell (CD45R/B220+) counts. No significant differences between groups A and B were observed on day 0; significant differences between the two groups observed thereafter are indicated with an asterisk (pMWU?<0.05). Significant differences over time (pF<0.05 and pD<0.05) and the corresponding significantly decreased or increased values are indicated with an arrow (black solid for group A and gray open for group B). In detail, increased T cell counts were observed in group A on days 2, 6 and 62 compared with day 83 and in group B on day 9 and 20 compared with day 83 (A). Decreased CD8+ T lymphocyte counts were observed in group A on day 20 compared with days 57 and 83 (C). In the CD4+/CD8+ ratio, in group A, significantly lower values were observed on day 48 compared to days 0 and 41, whereas in group B, higher counts were observed on day 0 compared to days 9 and 83 and on and 41 compared to day 9 (D). CD4+CD25+ cell counts varied significantly over time in cats in group B, with lower values on days 2, 9 and 27 compared with day 6 (E). B cells varied significantly over time in cats in group A, with transiently decreased values on days 20, 27, 34 and 41 compared with days 9, 62, 76 and 83 (F). No values were available for CD4+CD25+ on day 83.

From: Baumann J, Novacco M, Willi B, Riond B, Meli ML, Boretti FS, Hofmann-Lehmann R.
Lack of cross-protection against Mycoplasma haemofelis infection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.
Vet Res. 2015 Sep 24;46:104.

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CD4 Antibody | vpg34 gallery image 2

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FITC conjugated Mouse anti Feline CD4 antibody, clone vpg34 (MCA1346F) used to evaluate circulating CD4 expressing T lymphocyte prevelence in Feline immunodeficiency virus infected cat blood by flow cytometry.
Image caption:
Plasma viremia (a), proviral load in the PBMCs (b), and circulating CD4+ T lymphocyte percentages (c) of the study cats at the times indicated relative to the first FIV-MDC inoculum. CD4+ T cells were monitored by flow cytometry in peripheral blood by direct staining with anti-feline CD4-PE (clone vpg34, AbD Serotec, Raleigh, NC) for 30 min as previously described (8). Symbols represent individual animals. Arrows indicate the times of FIV-MDC inoculation.

From: Freer G, Matteucci D, Mazzetti P, Tarabella F, Ricci E, Bozzacco L, Merico A, Pistello M, Ceccherini-Nelli L, Bendinelli M.
Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
Retrovirology. 2008 Apr 17;5:33.

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Published customer image:
Mouse anti Feline CD4 antiody, clone vpg34 (MCA1346F) used for the evaluation of CD4 expressing T cell prevelence in peripheral blood by flow cytometry.
Image caption:
Immunological alterations following FIV infection.
Cats in Group 1 were challenged with clonal GL8 (variant B32), cats in group 2 were challenged with the reconstituted quasispecies challenge (B14, B19, B28, B30, B31 and B32) and control cats remained unchallenged. Percentages of lymphocytes expressing (A) CD4 and (B) CD8 were measured by flow cytometry and are shown as group means +/- SE. (C) CD4:CD8 ratios were calculated from the percentage of CD4 and CD8 positive lymphocytes and are displayed as group means +/- SE. p-values for statistically significant differences between infected and control groups are shown (Student's t-test).

From: Willett BJ, Kraase M, Logan N, McMonagle E, Varela M, Hosie MJ (2013)
Selective Expansion of Viral Variants following Experimental Transmission of a Reconstituted Feline Immunodeficiency Virus Quasispecies.
PLoS ONE 8(1): e54871.

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CD4 Antibody | vpg34 gallery image 4

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Mouse anti Feline CD4 antibody, clone vpg34 (MCA1346F) used to assess changes in CD4 expressing T lymphocytes in cat blood following Mycoplasma heamofelis infection, by flow cytometry.
Image caption:
CD4+ , CD8+ and CD5+ T lymphocytes, B lymphocytes and activated CD4+ T lymphocytes during the course of Mhf infection. Mean (±SD) cell counts of the CD4+ (A), CD8+ (B) and CD5+ T lymphocytes (C), CD4+/CD8+ ratio (D), B lymphocytes (CD45R/B220+, E) and activated CD4+ T lymphocytes (CD4+CD25+, F) at day 0 and during the 100-day pi period in group A (black dots, solid line) and group B (open triangles, dashed line). Cats were subcutaneously inoculated with Mhf at day 0. The onset of bacteremia in group A and B (day 7 pi, both groups) is indicated with a triangle. Boxes represent the time of peak bacteremia in group A (grey box) and B (dotted box). Statistically significant differences between group A and B are indicated with asterisks (pMWU<0.05). A CD4+ T lymphocyte counts changed significantly over time in group A (pF = 0.0012, lower at day 21 pi compared with days 3, 70 and 98 pi, pD<0.05). B CD8+ T lymphocyte counts changed significantly over time in group A (pF = 0.0002; lower at day 21 pi compared with days 3, 70 and 98 pi, pD<0.05). C CD5+ T lymphocyte counts changed significant over time in group A (pF<0.0002, lower at day 21 pi compared with days 3, 70 and 98 pi, pD<0.05). D CD4+/CD8+ ratio changed significantly over time in group A (pF = 0.0002, lower at days 14 and 21 pi compared with day 0 and day 98 pi, pD<0.05). E B lymphocyte counts changed significantly over time in group A (pF = 0.0103, lower at day 21 pi compared with day 3 pi, pD<0.05). F Activated CD4+ T lymphocyte counts changed significantly over time in group A (pF<0.0001, higher at days 3, 14 and 21 pi when compared with days 42, 56, 70 and 98 pi, pD<0.05) and group B (pF = 0.0249, no significance in the post test).

From: Sugiarto S, Spiri AM, Riond B, Novacco M, Oestmann A, de Miranda LH, Meli ML, Boretti FS, Hofmann-Lehmann R, Willi B.
Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis.
Vet Res. 2016 Aug 5;47(1):79.

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  • Mouse anti Cat CD4:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    vpg34
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1346FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1346F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Cat CD4 antibody, clone vpg34 recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes.
    • Intended Use
    • Target Species
      Cat
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Immunoaffinity purified feline CD4.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c were fused with cells of the NS0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : FITC vpg34 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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