Jurkat cells were treated as shown with 6 μM Camptothecin to induce apoptosis. The cells were then stained with SR FLICA Caspase-9 Kit (ICT961) and ReadiDrop™ Propidium Iodide (1351101). Apoptotic cells positive for caspases can be seen in the bottom right quadrant and dead cells positive for both caspase and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5™ Cell Analyzer.
The SR FLICA™ Caspase 9 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Approx. Protein Concentrations
Reagents In The Kit
4 vials of SR-LEHD-FMK FLICA™ Reagent - lyophilized 10X Wash Buffer, 60 mL Fixative, 6 mL Hoechst Stain, 1 mL
Preparing The Antibody
Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.
The Caspase FLICA™ Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Store the unopened kit (and each unopened component) at +4oC until the expiration date. Protect the FLICA™ reagent from light at all times.
Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light.
Please see label for expiry date.
FLICA™ is a trademark of Immunochemistry Technologies, LLC.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.