Brefeldin A Solution

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Figure A. RPE conjugated mouse anti human CD3 (MCA2184PE) and FITC conjugated mouse anti human IFN-gamma (MCA1581F) on unstimulated human lymphocytes. Figure B. RPE conjugated mouse anti human CD3 (MCA2184PE) and FITC conjugated mouse anti human IFN-gamma (MCA1581F) on human lymphocytes stimulated with PMA and ionomycin for 5 hours in the presence of brefeldin A (BUF075). Data acquired on the ZE5™ Cell Analyzer.

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  • Brefeldin A Solution (1000X)
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF075Fdatasheet pdfdatasheet pdf1 ml
    BUF075
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Brefeldin A Solution (1000x) is commonly used to enhance intracellular cytokine staining by inhibiting protein transport. This solution causes many of the cytokines to accumulate in the Golgi apparatus and endoplasmic reticulum.
    • Intended Use
    • Product Form
      1000x concentrate - liquid
    • Reconstitution
    • Preparation
      5 mg/ml solution prior to dilution
    • Preservative Stabilisers
      Dimethyl sulfoxide (DMSO)
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Thaw before use as DMSO freezes at the recommended storage conditions
      2. Dilute the Brefeldin A Solution (1000x) to a 1x working concentration using cell culture media
      3. Add Brefeldin A Solution (1x) to cell culture for 4-24 hours prior to antibody staining. Do not exceed cell culture time as this may affect cell viability

      Note: Aliquot product to avoid repeat freeze-thawing. Time and culture conditions may need to be optimized.

    Additional Brefeldin A Solution Formats

    Formats Applications Sizes available
    Brefeldin A Solution : Reagent F 1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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