Western blot using HSPA8 primary antibodyEnlarge
Western blot analysis was performed by loading 25 μl Hela lysate (lanes 2 and 3) and Precision Plus Protein™ Prestained Standards (lane 1) onto a AnykD™ Criterion™ TGX Stain-Free™ Protein Gel. The gel was run under reducing conditions and proteins were transferred to a nitrocellulose membrane. Lane 2 was probed with Anti-HSPA8 Antibody, clone AbD28028 Fab-A-FH format, at a concentration of 10 μg/ml. Lanes 2 and 3 were then probed with Human Anti-Bacterial Alkaline Phosphatase:HRP (clone AbD25296_hIgG2, HCA275P) at dilution of 1/1000. Visualization was carried out using the ChemiDoc™ MP Imaging System with an auto exposure. The Western blot bands (red) and protein standards (green) have been pseudocolored. Fab-A-FH format is a bivalent bacterial alkaline phosphatase fusion antibody (DYKDDDDK- and His-6-tags).