Varicella Zoster Virus

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Native Varicella Zoster Virus

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PIP023 E 1 ml
Native Varicella zoster Virus preparation contains a high concentration of virus and viral components as well as some cellular material suspended in EMEM with some serum proteins.

Varicella zoster virus is a member of the herpesvirus family. It commonly causes chickenpox in children and shingles and postherpetic neuralgia in adults. Chickenpox is a common childhood disease characterised by a vesicular skin rash, becoming itchy pockmarks which eventually heal, mostly without scarring. The disease has a 10-21 day incubation period and is easily spread through coughs and sneezes or direct contact with secretions from the rash. The disease is rare, but generally more serious, in adults. The primary outbreak usually confers lifelong protective immunity, however, the virus can go on to cause shingles, a completely different disease, many years after a chickenpox outbreak. Shingles is characterised by a painful skin rash in a limited area on the body. The rash normally heals within two to four weeks, but some individuals may suffer from postherpetic neuralgia, or nerve pain, for months or years to follow.

Product Details

Target Species
Viral
Product Form
Inactivated Varicella zoster virus
Preparation
Optimally infected monolayers are harvested, resuspended in a small volume of tissue culture fluid and disrupted by sonication. The suspension is subjected to low speed centrifugation and the resulting supernatant constitutes the antigen preparation.

The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.
Source
Varicella zoster virus, VZ-10 strain, cultured in MRC-5 cells.
Buffer Solution
Eagle's Minimum Essential Medium (MEM)
Preservative Stabilisers
None present
Activity
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
2.9 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Varicella Zoster Virus

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
PIP023 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.