Alkaline Phosphatase

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  • Native Calf Alkaline Phosphatase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0300-1706Edatasheet pdfdatasheet pdf5 mg
    0300-1706
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Product Form
      Purified AP from calf intestine - liquid
    • Reconstitution
    • Preparation
      Crystalline suspension. Free of endonuclease, exonuclease and RNase activities.
    • Preservative Stabilisers
      None present
    • Activity
      4000 U/mg protein.
      1 unit liberates 1 µmole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 27.44 mg/ml
    • Molecular Weight
      MW: 110 - 150 kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      70% Ammonium sulphate.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      alkaline phosphatase activity
      integral to membrane
      metal ion binding
      anchored to membrane
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      The product may be diluted in sterile distilled water or in dilute buffer.

    Additional Alkaline Phosphatase Formats

    Formats Applications Sizes available
    Alkaline Phosphatase : Purified E 5 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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