Crystalline suspension. Free of endonuclease, exonuclease and RNase activities.
4000 U/mg protein. 1 unit liberates 1 µmole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate.
Approx. Protein Concentrations
Total protein concentration 27.44 mg/ml
MW: 110 - 150 kD
Reagents In The Kit
Preparing The Antibody
70% Ammonium sulphate.
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Instructions For Use
The product may be diluted in sterile distilled water or in dilute buffer.