alamarBlue®

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alamarBlue® indicator dye (BUF012B) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring

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Published customer image:
TDP-43 pathological and glycine to proline mutant peptides exhibit different neurotoxicity. (A) The comparison of cell viability in the presence of D1, G294V, G295S, GGG294PPP, and GGG308PPP for 72 hours determined by AlamarBlue assay. Results were means ± SEM of three independent experiments (* = p<0.05, ** = p<0.01, N.S. = not significant). (B) Time-lapse DIC microscopy of N2a cell after the addition of PBS, GGG308PPP, or G295S for 36 hours.

From: Sun C-S, Wang CY-H, Chen BP-W, He R-Y, Liu GC-H, et al. (2014) The Influence of Pathological Mutations and Proline Substitutions in TDP-43 Glycine-Rich Peptides on Its Amyloid Properties and Cellular Toxicity. PLoS ONE 9(8): e103644.

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Published customer image:
AlamarBlue used for the determination of proliferation of pigeon lymphocytes in vitro in response to mitogens.
Image caption:
Experiment 2. Lymphocyte proliferation (% increase of mitogen-stimulated cells relative to un-stimulated cells) on y-axis, stimulated by (a) ConA at 5 μg ml-1, (b) LPS at 2.5 μg ml-1 and (c) PMA at 5 μg ml-1 on pigeons fed the 6, 10 and 14% crude protein (CP) diets on x-axis at 4 (black) and 8 (grey) hour incubation after adding alamarBlue® (mean?±?s.e., n?=?17: 6 and 10%; n?=?16: 14%). Different lower-case letters within the same hour indicate significant differences at p?<?0.05 using Tukey's post hoc test.

From: Mabuchi, Y. and Frankel, T.L.
Functions of innate and acquired immune system are reduced in domestic pigeons (Columba livia domestica) given a low protein diet. Royal Society Open Science 2016. 3: 150408.

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alamarBlue® gallery image 4

Published customer image:
AlamerBlue® used for the assessment of tumor slice viability in vitro using fluorescence readout.
Image caption:
FAK inhibition reduces cell viability in human lung tumor tissue but not normal lung tissue ex vivo. Tissues from five unselected patients were treated for 48 hours with vehicle control (DMSO), erlotinib (10 μM), PF-271 (5 μM), or the combination of erlotinib and PF-271. Cell viability was assessed for both tumor tissue and normal tissue by alamarBlue from three wells per condition with each well containing three 2 mm x 1 mm tissue pieces. Fluorescence was normalized to baseline fluorescence readings for each well taken prior to drug treatment. Experimentation was performed independently on each patient sample Mean fluorescence is indicated for each condition as a horizontal line and statistically significant differences were determined by ANOVA and indicated as P values above the comparison.

From: Howe GA, Xiao B, Zhao H, Al-Zahrani KN, Hasim MS, Villeneuve J, et al. (2016)
Focal Adhesion Kinase Inhibitors in Combination with Erlotinib Demonstrate Enhanced Anti-Tumor Activity in Non-Small Cell Lung Cancer.
PLoS ONE 11(3): e0150567.

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alamarBlue® gallery image 5

Published customer image:
AlamarBlue® used to evaluate murine pancreatic acinar cell viability in the presence of stimuli following in vitro culture.
Image caption:
Analysis of viability of pancreatic acinar cells in culture. Cell viability under the different treatments applied was analyzed studying AlamarBlue reduction, as described in “Material and methods”. (A) Incubation of cells in the presence of 1 nM CCK-8 did not alter cell viability significantly along the incubation period. (B) Effect of incubation of cells with 1 µM Tps on cell viability. (C) H2O2 (100 µM) led to a decrease in cell viability compared to non-stimulated cells. The horizontal dotted line represents the viability of non-stimulated, which was considered 100%. Graphics are representative of 4 independent experiments for each treatment (*, P< 0.001 for treatments vs non-stimulated cells).

From: Santofimia-Castaño, P. et al.Change in the Characteristics of Ca2+ Signaling in Pancreatic Acinar Cells in Culture. Santofimia-Castaño, P. et al.
Change in the Characteristics of Ca2+ Signaling in Pancreatic Acinar Cells in Culture.
The Open Access Journal of Science and Technology Vol. 2 (2014), Article ID 101058

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  • alamarBlue®
  • alamarBlue®
(Rated 4.0 out of 5 based on 1 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF012BE, FN, IFdatasheet pdfdatasheet pdf100 ml
    BUF012B
    BUF012AE, FN, IFdatasheet pdfdatasheet pdf25 ml
    BUF012A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • The cell proliferation assay reagent alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.

      alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.

      Some variability in the absorbance may occur between batches of AlamarBlue® but all batches should fall between 0.84 and 0.95AU when measured between 600nm and 602nm on a spectrophotometer.

      Full cell proliferation assay instructions can be found at
      www.bio-rad-antibodies.com/uploads/IFU/BUF012A.pdf

      For further information and Technical help about alamarBlue®, the cell proliferation assay reagent, please visit www.bio-rad-antibodies.com/alamarBlue
      This site includes:
      Frequently Asked Questions
      Example calculations
      Product-related references
    • Intended Use
      • Cell proliferation assays.
      • The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
      • Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
      • alamarBlue® is for use between pH6.8 and pH7.4.

      • Cell proliferation assays.
      • The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
      • Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
      • alamarBlue® is for use between pH6.8 and pH7.4.

    • Product Form
      Liquid
      Liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present
      None present
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
      Cell proliferation assay.
      • Growing cells cause a chemical reduction of alamarBlue®.
      • Continued growth maintains a reduced environment. (fluorescent, red).
      • Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
      • Data may be collected using either fluorescence-based or absorbance-based instrumentation.
      • Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
      • Absorbance is monitored at 570nm and 600nm.
      Cell proliferation assay
      • Growing cells cause a chemical reduction of alamarBlue®.
      • Continued growth maintains a reduced environment. (fluorescent, red).
      • Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
      • Data may be collected using either fluorescence-based or absorbance-based instrumentation.
      • Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
      • Absorbance is monitored at 570nm and 600nm.
    • Buffer Solution
    • Storage
      This product may be stored for 12 months at room temperature.
      Store at 2-8oC to increase the shelf life to 20 months.

      This product should be stored undiluted.
      This product is photosensitive and should be protected from light.

      This product may be stored for 12 months at room temperature.
      Store at 2-8oC to increase the shelf life to 20 months.

      This product should be stored undiluted.
      This product is photosensitive and should be protected from light.

    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.
      Manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/BUF012A.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/BUF012B.pdf

    Additional alamarBlue® Formats

    Formats Applications Sizes available
    alamarBlue® : Reagent E, FN, IF 25 ml | 100 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          alamarBlue®BUF012B100 mlE, FN, IF
          BUF012B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          alamarBlue®BUF012A25 mlE, FN, IF
          BUF012A

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

              Write your review

              Good value
              4/5stars
              by
              Submitted: 6 Jul 2016
              Product works as well as other products on the market for less money.

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