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5x ELISA Coating Buffer
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- 5x ELISA Coating Buffer
- 5x ELISA Coating Buffer
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Product TypeAccessory Reagent
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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Intended UsePack Size: 100 mlBUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 500 mlBUF030B is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 1000 mlBUF030C is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 100 mlBUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 500 mlBUF030B is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 1000 mlBUF030C is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 100 mlBUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 500 mlBUF030B is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.Pack Size: 1000 mlBUF030C is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage. -
Product Form5 x concentrate - liquid5 x concentrate - liquid5 x concentrate - liquid
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Reconstitution
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Preparation
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Preservative Stabilisers
0.5% Sodium Azide 0.5% Sodium Azide 0.5% Sodium Azide -
Purity
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Approx. Protein Concentrations
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered salinePhosphate buffered saline
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StoragePack Size: 100 mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.Pack Size: 500 ml, 1000 mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.Pack Size: 100 mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.Pack Size: 500 ml, 1000 mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.Pack Size: 100 mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.Pack Size: 500 ml, 1000 mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil. -
Shelf Life24 months from date of despatch.24 months from date of despatch.24 months from date of despatch.
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Application Name Yes No Min Dilution Max Dilution ELISA Application Name Yes No Min Dilution Max Dilution ELISA - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
- Technical Advice
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Western Blotting
- Instructions For Use1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation. - Instructions For Use1. Dilute the required volume of BUF030C 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation. - Instructions For Use1. Dilute the required volume of BUF030B 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
Additional 5x ELISA Coating Buffer Formats
| Formats | Applications | Sizes available |
|---|---|---|
| 5x ELISA Coating Buffer : 5 X Concentrate | E | 100 ml | 1000 ml | 500 ml |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| 10x ELISA Wash Buffer | BUF031A | 100 ml | E | BUF031A | |
| ELISA BSA Block | BUF032A | 100 ml | E | BUF032A |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| 10x ELISA Wash Buffer | BUF031A | 100 ml | E | BUF031A | |
| ELISA BSA Block | BUF032A | 100 ml | E | BUF032A |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| 10x ELISA Wash Buffer | BUF031A | 100 ml | E | BUF031A | |
| ELISA BSA Block | BUF032A | 100 ml | E | BUF032A |
Recommended Positive Controls
Histology Controls
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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