5x ELISA Coating Buffer

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Product Type

Accessory Reagent

1 Formats Available

Product Code	Applications	Pack Size	List Price
BUF030A	E	100 ml	BUF030A
BUF030C	E	1000 ml	BUF030C
BUF030B	E	500 ml	BUF030B

Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Intended Use

Pack Size: 100 mlBUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 500 mlBUF030B is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 1000 mlBUF030C is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 100 mlBUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 500 mlBUF030B is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 1000 mlBUF030C is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 100 mlBUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 500 mlBUF030B is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Pack Size: 1000 mlBUF030C is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
Product Form

5 x concentrate - liquid
5 x concentrate - liquid
5 x concentrate - liquid
Reconstitution
Preparation
Preservative Stabilisers

0.5% Sodium Azide
0.5% Sodium Azide
0.5% Sodium Azide
Purity
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Test Principle
Buffer Solution

Phosphate buffered saline
Phosphate buffered saline
Phosphate buffered saline
Storage

Pack Size: 100 mlStore at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 1000 mlStore at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 100 mlStore at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 1000 mlStore at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 100 mlStore at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 1000 mlStore at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Shelf Life

24 months from date of despatch.
24 months from date of despatch.
24 months from date of despatch.
Acknowledgements
Regulatory

For research purposes only

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Yes	No	Min Dilution	Max Dilution
ELISA

Application Name	Yes	No	Min Dilution	Max Dilution
ELISA

Application Name	Yes	No	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.

Technical Advice

Recommended Protocol

ELISA

Immunohistology

Histology Positive Control Tissue

Immunofluorescence

Western Blotting

Instructions For Use

1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

Instructions For Use

1. Dilute the required volume of BUF030C 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

Instructions For Use

1. Dilute the required volume of BUF030B 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.