10x ELISA Wash Buffer

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  • 10x ELISA Wash Buffer
  • 10x ELISA Wash Buffer
  • 10x ELISA Wash Buffer
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF031AEdatasheet pdfdatasheet pdf100 ml
    BUF031A
    BUF031CEdatasheet pdfdatasheet pdf1000 ml
    BUF031C
    BUF031BEdatasheet pdfdatasheet pdf500 ml
    BUF031B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      Pack Size: 100 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031A is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 500 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031B is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 1000 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031C is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 100 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031A is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 500 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031B is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 1000 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031C is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 100 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031A is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 500 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031B is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
      Pack Size: 1000 mlELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

      BUF031C is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
    • Product Form
      Pack Size: 100 ml, 1000 ml10 x concentration - liquid
      Pack Size: 500 ml10x concentrate - liquid
      Pack Size: 100 ml, 1000 ml10 x concentration - liquid
      Pack Size: 500 ml10x concentrate - liquid
      Pack Size: 100 ml, 1000 ml10 x concentration - liquid
      Pack Size: 500 ml10x concentrate - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      1.0% 2-chloroacetamide
      1.0% 2-chloroacetamide
      1.0% 2-chloroacetamide
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 100 ml, 500 mlStore at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Pack Size: 1000 mlStore at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Pack Size: 100 ml, 500 mlStore at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Pack Size: 1000 mlStore at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Pack Size: 100 ml, 500 mlStore at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Pack Size: 1000 mlStore at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
    • Shelf Life
      24 months from date of despatch.
      24 months from date of despatch.
      24 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1. Dilute the required volume of BUF031A 1:10 with deionised water (1 part BUF031A to 9 parts water) and mix.

      2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

      3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
    • Instructions For Use
      1. Dilute the required volume of BUF031B 1:10 with deionised water (1 part BUF031B to 9 parts water) and mix.

      2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

      3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
    • Instructions For Use
      1. Dilute the required volume of BUF031C 1:10 with deionised water (1 part BUF031C to 9 parts water) and mix.

      2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

      3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.

    Additional 10x ELISA Wash Buffer Formats

    Formats Applications Sizes available
    10x ELISA Wash Buffer : Concentrate E 100 ml | 500 ml | 1000 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A

          Recommended Positive Controls

            Histology Controls

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