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Recombinant Dengue type 4 NS1 protein (PIP050A) and recombinant Dengue type 1 NS1 (PIP047A) protein used to assess specificity of generated mouse anti NS1 antibody clones by ELISA.
ELISAs showing humoral immune responses of mice immunized with D1NS1-HEK and D4NS1-HEK cells. a and b: Mouse antisera of D1NS1-HEK (red, panel a) and D4NS1-HEK (blue, panel b) immunized mouse groups (4 mice each) were analyzed on recombinant D1NS1 and recombinant D4NS1, respectively. c and d: Serotype cross-reactive humoral immune responses of D1NS1-HEK (red, panel c) and D4NS1-HEK (blue, panel d) mouse groups were analyzed on recombinant D4NS1 and recombinant D1NS1, respectively. Blue line (panel c) and red line (panel d)?=?pool of D4NS1-HEK and D1NS1-HEK mouse antisera, respectively, to enable direct comparison with the level of serotype-specific immune responses. e and f: Analysis of pooled mouse sera (red?=?D1NS1-HEK (e), blue?=?D4NS1-HEK (f)) on an unrelated hexa-His tagged protein (MUL_3720) as a control for humoral responses against the hexa-His tag. Black line in all panels?=?pool of mouse sera before immunization (pre-bleed)
From: Rose N, Pinho-Nascimento CA, Ruggieri A, Favuzza P, Tamborrini M, Roth H, Baroni de Moraes MT, Matile H, Jänisch T, Pluschke G, Röltgen K.
Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization.
BMC Biotechnol. 2016 Nov 22;16(1):83.