Dengue Virus Type 4 NS1 Antigen

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Dengue Virus Type 4 NS1 Antigen gallery image 1

Published customer image:
Recombinant Dengue type 4 NS1 protein (PIP050A) and recombinant Dengue type 1 NS1 (PIP047A) protein used to assess specificity of generated mouse anti NS1 antibody clones by ELISA.
Image caption:
ELISAs showing humoral immune responses of mice immunized with D1NS1-HEK and D4NS1-HEK cells. a and b: Mouse antisera of D1NS1-HEK (red, panel a) and D4NS1-HEK (blue, panel b) immunized mouse groups (4 mice each) were analyzed on recombinant D1NS1 and recombinant D4NS1, respectively. c and d: Serotype cross-reactive humoral immune responses of D1NS1-HEK (red, panel c) and D4NS1-HEK (blue, panel d) mouse groups were analyzed on recombinant D4NS1 and recombinant D1NS1, respectively. Blue line (panel c) and red line (panel d)?=?pool of D4NS1-HEK and D1NS1-HEK mouse antisera, respectively, to enable direct comparison with the level of serotype-specific immune responses. e and f: Analysis of pooled mouse sera (red?=?D1NS1-HEK (e), blue?=?D4NS1-HEK (f)) on an unrelated hexa-His tagged protein (MUL_3720) as a control for humoral responses against the hexa-His tag. Black line in all panels?=?pool of mouse sera before immunization (pre-bleed)

From: Rose N, Pinho-Nascimento CA, Ruggieri A, Favuzza P, Tamborrini M, Roth H, Baroni de Moraes MT, Matile H, Jänisch T, Pluschke G, Röltgen K.
Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization.
BMC Biotechnol. 2016 Nov 22;16(1):83.

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  • Recombinant Dengue Virus Type 4 NS1 Antigen
  • Recombinant Dengue Virus Type 4 NS1 Antigen
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP050AEdatasheet pdfdatasheet pdf100 µg
    PIP050A
    PIP050BEdatasheet pdfdatasheet pdf500 µg
    PIP050B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant dengue virus type 4 NS1 antigen is a purified preparation of the dengue virus serotype 4 (DENV4), non-structural protein 1 (NS1). DENV4 is one of four (DENV1-4) antigenically distinct, closely related viral serotypes belonging to the Flaviviridae family, genus flavivirus. All four serotypes are known to cause viral infection in humans.

      Dengue virus is common mosquito-borne infection and a major cause of morbidity in tropical and subtropical regions. There is currently no vaccine to prevent, or effective anti-viral drugs to treat, dengue virus infection. In many cases infection is assymptomatic and the majority of individuals who get ill only suffer the mild, non-specific febrile symptoms characteristic of dengue fever (DF). Only a minority of infections result in severe disease, manifesting as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue virus infection gives lifelong immunity to the serotype in question but subsequent infection with another serotype may increase the likelihood of severe disease.

      The NS1 glycoprotein is essential for viral replication and viability, and since this protein is secreted into the bloodstream, tests have been developed to diagnose DENV infections using NS1, including antigen-capture ELISA, lateral flow antigen detection, and the measurement of NS1-specific IgM and IgG responses (Guzman, M.G. et al. 2010).

      Recombinant DENV4 NS1 antigen is presented in its native folded state complete with post-translational modifications, delivering optimal antigenicity and making it suitable for use in vaccine research and serology-based assays.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified recombinant protein with a C-terminal His-tag - liquid
      Purified recombinant protein with a C-terminal His-tag - liquid
    • Reconstitution
    • Preparation
      Recombinant dengue virus serotype 4 NS1 protein, sequence strain Dominica/814669/1981, expressed in 293 human cells
      Recombinant dengue virus serotype 4 NS1 protein, sequence strain Dominica/814669/1981, expressed in 293 human cells
    • Preservative Stabilisers
      None present
      None present
    • Purity
      >90% by SDS PAGE analysis
      >90% by SDS PAGE analysis
    • Approx. Protein Concentrations
      Pack Size: 100 µgApproximate protein concentration 0.55 mg/ml
      Pack Size: 500 µgApproximate protein concentration 0.5 mg/ml
      Pack Size: 100 µgApproximate protein concentration 0.55 mg/ml
      Pack Size: 500 µgApproximate protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Tris buffered saline
      Tris buffered saline
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Dengue Virus Type 4 NS1 Antigen Formats

    Formats Applications Sizes available
    Dengue Virus Type 4 NS1 Antigen : Rec. Protein E 500 µg | 100 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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