Cytomegalovirus

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  • Native Cytomegalovirus
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  • Product Type
    Antigen
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP005Edatasheet pdfdatasheet pdf1 ml
    PIP005
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Cytomegalovirus is a member of the Herpesviridae family. It may be transmitted from person to person through urine, saliva, other bodily fluids, breast milk and transplanted organs. It is a common infection which mostly passes unnoticed. The infection may be dangerous to people who are immunocompromised such as those who are HIV positive or organ transplant recipients. The infection may also be dangerous to pre- and post-natal infants. Antiviral drugs are useful in the treatment of CMV infection.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Inactivated Cytomegalovirus - liquid
    • Reconstitution
    • Preparation
      MRC-5 monolayers are infected in a manner which will ensure that the full host of viral proteins are present upon harvest. Optimally infected tissue is collected in glycine buffered saline. The cells are disrupted to release cellular CMV antigen. Cellular debris is removed by centrifugation.

      The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.
    • Source
      Cytomegalovirus strain AD-169, cultured in MRC-5, a diploid cell line of human lung origin.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 3.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      PIP005 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.

    Additional Cytomegalovirus Formats

    Formats Applications Sizes available
    Cytomegalovirus : Inactivated Pathogen E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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