RPE-Cy7 Conjugation Kit

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  • LYNX Rapid RPE-Cy7 Antibody Conjugation Kit
  • LYNX Rapid RPE-Cy7 Antibody Conjugation Kit
  • LYNX Rapid RPE-Cy7 Antibody Conjugation Kit
  • LYNX Rapid RPE-Cy7 Antibody Conjugation Kit
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  • Product Type
    Conjugation Kit
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    LNK111PECY7CJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf1 Conjugation For 60µg Antibody
    LNK111PECY7
    LNK113PECY7CJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf1 Conjugation For 600µg Antibody
    LNK113PECY7
    LNK114PECY7CJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 10µg Antibody
    LNK114PECY7
    LNK112PECY7CJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 60µg Antibody
    LNK112PECY7
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • LYNX Rapid RPE-Cy7 Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing R-Phycoerythrin (RPE)-Cy7 label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of RPE-Cy7 to the antibody.

      The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Pack Size: 1 Conjugation For 60µg Antibody1 Vial of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 60µg Antibody3 Vials of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 600µg Antibody1 Vial of 1mg LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 60µg Antibody1 Vial of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 60µg Antibody3 Vials of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 600µg Antibody1 Vial of 1mg LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 60µg Antibody1 Vial of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 60µg Antibody3 Vials of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 600µg Antibody1 Vial of 1mg LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 60µg Antibody1 Vial of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 60µg Antibody3 Vials of 100ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 600µg Antibody1 Vial of 1mg LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized RPE-Cy7 mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
    • Preparing The Antibody
      Pack Size: 1 Conjugation For 60µg Antibody, 3 Conjugations For 60µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 40ul, although up to 80ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 600µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 400ul, although up to 800ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label the quantity of RPE-tandem dye is in slight molar excess. Approximately 10ug of IgG will give a 1:1 molar ratio of antibody:RPE. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 60µg Antibody, 3 Conjugations For 60µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 40ul, although up to 80ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 600µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 400ul, although up to 800ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label the quantity of RPE-tandem dye is in slight molar excess. Approximately 10ug of IgG will give a 1:1 molar ratio of antibody:RPE. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 60µg Antibody, 3 Conjugations For 60µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 40ul, although up to 80ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 600µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 400ul, although up to 800ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label the quantity of RPE-tandem dye is in slight molar excess. Approximately 10ug of IgG will give a 1:1 molar ratio of antibody:RPE. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 60µg Antibody, 3 Conjugations For 60µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 40ul, although up to 80ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 600µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label, it is recommended that 50-60ug of antibody be used for every 100ug RPE-Cy7, to ensure a slight RPE-Cy7 molar excess (50ug antibody gives a 1:1 Ab:RPE-Cy7 molar ratio). For optimal results the antibody volume should be around 400ul, although up to 800ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      Due to the large size of the RPE-Cy7 label the quantity of RPE-tandem dye is in slight molar excess. Approximately 10ug of IgG will give a 1:1 molar ratio of antibody:RPE. For optimal results the antibody volume should be around 4-10ul.
    • Test Principle
    • Buffer Solution
    • Storage
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
    • Licensed Use
      These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: antibodies@bio-rad.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation


    • We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2.Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3.Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
    • Instructions For Use
      1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2.Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3.Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
    • Instructions For Use
      1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2.Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3.Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
    • Instructions For Use
      1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2.Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3.Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    Additional RPE-Cy7 Conjugation Kit Formats

    Formats Applications Sizes available
    RPE-Cy7 Conjugation Kit : Kit CJ 3 Conjugations For 60µg Antibody | 1 Conjugation For 600µg Antibody | 3 Conjugations For 10µg Antibody | 1 Conjugation For 60µg Antibody
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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