Rat IgG2b Negative Control Antibody

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Rat IgG2b Negative Control Antibody gallery image 1

Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat IgG2b isotype control (MCA6006F). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat anti Human CD18 (MCA503F). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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Rat IgG2b Negative Control Antibody gallery image 2

Figure A. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat IgG2b isotype control (MCA6006PE). Figure B. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat anti Human CD28 (MCA709PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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Rat IgG2b Negative Control Antibody gallery image 3

Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 PE (STAR74D649) and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat IgG2b Negative Control Antibody thumbnail image 1
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  • Rat IgG2b Negative Control
  • Rat IgG2b Negative Control:Alexa Fluor® 488
  • Rat IgG2b Negative Control
  • Rat IgG2b Negative Control:FITC
  • Rat IgG2b Negative Control:Biotin
  • Rat IgG2b Negative Control:RPE
  • Rat IgG2b Negative Control:APC
  • Rat IgG2b Negative Control:Alexa Fluor® 647
  • Rat IgG2b Negative Control:Pacific Blue®
  • Rat IgG2b Negative Control:Low Endotoxin
  • Rat IgG2b Negative Control:RPE-Alexa Fluor® 750
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).
    Isotype controls savings up to 65%
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgG2b
    10 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA6006GAFdatasheet pdfdatasheet pdf0.1 mg
      MCA6006GA
      MCA6006FFdatasheet pdfdatasheet pdf0.1 mg
      MCA6006F
      MCA6006BFdatasheet pdfdatasheet pdf0.1 mg
      MCA6006B
      MCA6006ELFdatasheet pdfdatasheet pdf0.5 mg
      MCA6006EL
      MCA6006Fdatasheet pdfdatasheet pdf1 mg
      MCA6006
      MCA6006PEFdatasheet pdfdatasheet pdf100 Tests
      MCA6006PE
      MCA6006APCFdatasheet pdfdatasheet pdf100 Tests
      MCA6006APC
      MCA6006A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6006A488
      MCA6006A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6006A647
      MCA6006PBFdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6006PB
      MCA6006P750Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6006P750
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human cells in flow cytometry.

        Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor 488 - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG - lyophilized
        Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
        Purified IgG conjugated to Alexa Fluor 647 - liquid
        Purified IgG conjugated to Pacific Blue® - liquid
        Purified IgG - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE): Alexa Fluor® 750 - lyophilised
      • Reconstitution
        Reconstitute with 1.0 ml distilled water
        Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
        Reconstitute with 1.0 ml distilled water
        Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        None present
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        KLH
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC.
        After reconstitution store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC. DO NOT FREEZE.
        This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      • Shelf Life
        18 months from date of despatch
        18 months from date of despatch
        18 months from date of despatch.
        18 months from date of despatch
        18 months from date of despatch
        18 months from date of reconstitution
        18 months from date of reconstitution
        18 months from date of despatch
        18 months from date of despatch
        18 months from date of despatch
        18 months from date of despatch
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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