Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues.
We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitute with 0.5 ml distilled water
Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
Bovine Serum Albumin
Whole rat IgG.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 50ul of the suggested working dilution to label 106 cells in 100ul.
1. Bellingan. G. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.J. Exp. Med. 196:1515-1521.
2. Broderick, C.A. et al. (2005) Local Administration of an Adeno-associated Viral Vector Expressing IL-10 Reduces Monocyte Infiltration and Subsequent Photoreceptor Damage during Experimental Autoimmune UveitisMol Ther. 12: 369-73.
3. Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell–endothelium interactionsJ Anat. 214: 183-95.
4. Curran, C.S. et al. (2006) Lactoferrin activates macrophages via TLR4-dependent and -independent signaling pathwaysCell Immunol. 242: 23-30.
5. Guilloteau, L.A. et al. (2003) Nramp1 Is Not a Major Determinant in the Control of Brucella melitensis Infection in MiceInfect Immun. 71: 621-8.
6. Maemura, K. et al. (2006) Antigen-presenting cells expressing glutamate decarboxylase 67 were identified as epithelial cells in glutamate decarboxylase 67-GFP knock-in mouse thymus.Tissue Antigens. 67: 198-206.