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IgG Antibody

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Detection of Rat anti Mouse TREM2 (MCA4772) in a flow cytometric analysis of mouse TREM2 transfected cells using Goat F(ab')2 anti Rat IgG:RPE (STAR73)

  • IgG Antibody thumbnail image 1
  • Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR73Fdatasheet pdfdatasheet pdf0.5 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues.

      We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
    • Intended Use
    • Target Species
    • Product Form
      F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
    • Preparation
    • Antiserum Preparation
      Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Whole rat IgG.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 50ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : RPE F 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IgG Antibody thumbnail image 1


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