IgG Antibody

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  • Goat anti Rabbit IgG(Fc):HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5213-2504C, E, WBdatasheet pdfdatasheet pdf2 mg
    5213-2504
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rabbit IgG, rabbit IgG Fc and rabbit serum. No reaction was observed against rabbit IgG F(ab')2.
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Ig Fraction conjugated to Horseradish Peroxidase (HRP) - lyophilised
    • Reconstitution
      Use 1.0 ml of sterile DI water. For extended storage reconstitute with 50% glycerol instead of water and then aliquot and store at -20ºC. Avoid freeze thaw cycles.
    • Preparation
      Purified Ig prepared by affinity chromatography
    • Preservative Stabilisers
      0.01% Gentamicin sulphate
      10 mg/ml Bovine Serum Albumin
    • Immunogen
      Native
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/7000
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP C, E, WB 2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A

          Recommended Positive Controls

            Histology Controls

              References

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