Dengue Virus Type 4 NS1 Antigen
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|Recombinant dengue virus type 4 NS1 antigen is a purified preparation of the dengue virus serotype 4 (DENV4), non-structural protein 1 (NS1). DENV4 is one of four (DENV1-4) antigenically distinct, closely related viral serotypes belonging to the Flaviviridae family, genus flavivirus. All four serotypes are known to cause viral infection in humans.
Dengue virus is common mosquito-borne infection and a major cause of morbidity in tropical and subtropical regions. There is currently no vaccine to prevent, or effective anti-viral drugs to treat, dengue virus infection. In many cases infection is assymptomatic and the majority of individuals who get ill only suffer the mild, non-specific febrile symptoms characteristic of dengue fever (DF). Only a minority of infections result in severe disease, manifesting as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue virus infection gives lifelong immunity to the serotype in question but subsequent infection with another serotype may increase the likelihood of severe disease.
The NS1 glycoprotein is essential for viral replication and viability, and since this protein is secreted into the bloodstream, tests have been developed to diagnose DENV infections using NS1, including antigen-capture ELISA, lateral flow antigen detection, and the measurement of NS1-specific IgM and IgG responses (Guzman, M.G. et al. 2010).
Recombinant DENV4 NS1 antigen is presented in its native folded state complete with post-translational modifications, delivering optimal antigenicity and making it suitable for use in vaccine research and serology-based assays.
- Target Species
- Product Form
- Purified recombinant protein with a C-terminal His-tag - liquid
- Recombinant dengue virus serotype 4 NS1 protein, sequence strain Dominica/814669/1981, expressed in 293 human cells
- Buffer Solution
- Tris buffered saline
- Preservative Stabilisers
- None present
- >90% by SDS PAGE analysis
- Approx. Protein Concentrations
- Current, batch-specific concentration 0.63 mg/ml
- For research purposes only
- 12 months from date of despatch
- His-tag is a registered trademark of EMD Biosciences.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
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References for Dengue Virus Type 4 NS1 Antigen
Rose, N. et al. (2016) Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization.
BMC Biotechnol. 16 (1): 83.
Guzman, M.G. et al. (2010) Dengue: a continuing global threat.
Nat Rev Microbiol. 8 (12 Suppl): S7-16.
- DENV4 NS1 Antigen
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