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IL-1 Beta

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Recombinant Rat Interleukin-1 beta

Product Type
Recombinant Protein
Specificity
IL-1 Beta

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PRP23
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Recombinant rat interleukin-1 beta is an E. coli-derived recombinant protein corresponding to full-length mature rat interleukin 1 beta (IL-1b), an immunomodulary cytokine that mediates a wide range of immune responses.

Target Species
Rat
Product Form
Purified recombinant protein - lyophilized.
Reconstitution
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Escherichia coli.
Source
E.coli
Buffer Solution
10mM Sodium Acetate pH6.5
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
PRP23 has a specific activity of > 1 x 107 units/mg as determined by dose-dependent stimulation of proliferation of murine D10S cells.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight
17.3kD (153 Amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 10ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
This product may be used as the standard in ELISA applications with either AAR15G or AAR15B.
Western Blotting
This product may be used as the positive control in western blotting applications with either AAR15G or AAR15B.

UniProt
Q63264
Entrez Gene
Il1b
GO Terms
GO:0001660 fever generation
GO:0001666 response to hypoxia
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007568 aging
GO:0007613 memory
GO:0042493 response to drug
GO:0009408 response to heat
GO:0010193 response to ozone
GO:0010243 response to organic nitrogen
GO:0010332 response to gamma radiation
GO:0014050 negative regulation of glutamate secretion
GO:0019221 cytokine-mediated signaling pathway
GO:0030728 ovulation
GO:0032355 response to estradiol stimulus
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0032874 positive regulation of stress-activated MAPK cascade
GO:0033280 response to vitamin D
GO:0033591 response to L-ascorbic acid
GO:0035176 social behavior
GO:0042060 wound healing
GO:0043065 positive regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043278 response to morphine
GO:0043507 positive regulation of JUN kinase activity
GO:0046330 positive regulation of JNK cascade
GO:0051384 response to glucocorticoid stimulus
GO:0051781 positive regulation of cell division
GO:0070374 positive regulation of ERK1 and ERK2 cascade

PRP23

160465 161694 166529 166919

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