TNF Alpha

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Recombinant Mouse TNF Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP24 E FN WB 20 µg
Tumour necrosis factor alpha (TNFα) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumour cells and certain other target cells.

Product Details

Target Species
Mouse
Product Form
Purified protein produced in E. coli - lyophilised.
Reconstitution
Reconstitute with 0.2ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
10mM Sodium Phosphate
Preservative Stabilisers
None present
Activity
1 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
17.3kD (156 amino acid residues)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P06804 Related reagents
Entrez Gene
Tnf Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001891 phagocytic cup
GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0006006 glucose metabolic process
GO:0006927 transformed cell apoptosis
GO:0006959 humoral immune response
GO:0007254 JNK cascade
GO:0009887 organ morphogenesis
GO:0050830 defense response to Gram-positive bacterium
GO:0008625 induction of apoptosis via death domain receptors
GO:0009897 external side of plasma membrane
GO:0030198 extracellular matrix organization
GO:0030316 osteoclast differentiation
GO:0031622 positive regulation of fever generation
GO:0032729 positive regulation of interferon-gamma production
GO:0032755 positive regulation of interleukin-6 production
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0044130 negative regulation of growth of symbiont in host
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045121 membrane raft
GO:0045670 regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0045994 positive regulation of translational initiation by iron
GO:0046325 negative regulation of glucose import
GO:0046330 positive regulation of JNK cascade
GO:0051023 regulation of immunoglobulin secretion
GO:0051798 positive regulation of hair follicle development
GO:0055037 recycling endosome
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
GO:0060693 regulation of branching involved in salivary gland morphogenesis
Regulatory
For research purposes only

Applications of TNF Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.05ng/ml 20ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant mouse TNFα may be used as the standard for ELISA applications with anti-Mouse TNFα (purified) (AAM19GA).
Western Blotting
Recombinant mouse TNFα may be used as the positive control for western blotting applications with anti-Mouse TNFα (AAM19GA).