IL-10

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Recombinant Mouse Interleukin-10

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PMP21
Datasheet
E FN WB
SDS
10 µg loader

IL-10 has a range of biological properties, including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response.

ED50 is <2.0ng/ml, as determined by the dose dependent co-stimulation of MC/9 cells (with murine IL-4).

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein expressed in E. coli - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
5mM Sodium Phosphate
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
5 x 105 units/mg
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
18.7 kDa (161 amino acid sequence)
Endotoxin Level
< 0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
3 months from date of reconstitution

More Information

UniProt
P18893
Entrez Gene
Il10
GO Terms
GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus
GO:0042742 defense response to bacterium
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0030886 negative regulation of myeloid dendritic cell activation
GO:0032689 negative regulation of interferon-gamma production
GO:0032695 negative regulation of interleukin-12 production
GO:0032720 negative regulation of tumor necrosis factor production
GO:0044130 negative regulation of growth of symbiont in host
GO:0043193 positive regulation of gene-specific transcription
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0060670 branching involved in embryonic placenta morphogenesis
Regulatory
For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.2 20ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PMP21 may be used as a standard for ELISA applications with AAM32
Western Blotting
PMP21 may be used as a positive control for Western blotting applications with AAM32

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Mouse Interleukin-10 AAM32 E FN WB 0.1 mg loader

Product Specific References

References for IL-10

  1. Gelderblom, H. et al. (2007) Role of interleukin 10 during persistent infection with the relapsing fever Spirochete Borrelia turicatae.
    Am J Pathol. 170: 251-62.