IL-10
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IL-10 has a range of biological properties, including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response.
ED50 is <2.0ng/ml, as determined by the dose dependent co-stimulation of MC/9 cells (with murine IL-4).
ED50 is <2.0ng/ml, as determined by the dose dependent co-stimulation of MC/9 cells (with murine IL-4).
Product Details
- Target Species
- Mouse
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Buffer Solution
- 5mM Sodium Phosphate
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- 5 x 105 units/mg
- Purity
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 18.7 kDa (161 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P18893
- Entrez Gene
- Il10
- GO Terms
- GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus
- GO:0042742 defense response to bacterium
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0030886 negative regulation of myeloid dendritic cell activation
- GO:0032689 negative regulation of interferon-gamma production
- GO:0032695 negative regulation of interleukin-12 production
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0060670 branching involved in embryonic placenta morphogenesis
- Regulatory
- For research purposes only
Applications of IL-10
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Functional Assays | 0.2 | 20ng/ml | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse Interleukin-10 | AAM32 | E FN WB | 0.1 mg |
|
Product Specific References
References for IL-10
-
Gelderblom, H. et al. (2007) Role of interleukin 10 during persistent infection with the relapsing fever Spirochete Borrelia turicatae.
Am J Pathol. 170: 251-62.
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