IL-10

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Recombinant Mouse Interleukin-10

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PMP21 Datasheet	E FN WB SDS	10 µg	Log in		Get Quote

IL-10 has a range of biological properties, including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response.

ED50 is <2.0ng/ml, as determined by the dose dependent co-stimulation of MC/9 cells (with murine IL-4).

Product Details

Target Species: Mouse
Product Form: Purified recombinant protein expressed in E. coli - lyophilized
Reconstitution: Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution: 5mM Sodium Phosphate
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: 5 x 10⁵ units/mg
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 18.7 kDa (161 amino acid sequence)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: P18893
Entrez Gene: Il10
GO Terms: GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus; GO:0042742 defense response to bacterium; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0030886 negative regulation of myeloid dendritic cell activation; GO:0032689 negative regulation of interferon-gamma production; GO:0032695 negative regulation of interleukin-12 production; GO:0032720 negative regulation of tumor necrosis factor production; GO:0044130 negative regulation of growth of symbiont in host; GO:0043193 positive regulation of gene-specific transcription; GO:0045348 positive regulation of MHC class II biosynthetic process; GO:0060670 branching involved in embryonic placenta morphogenesis
Regulatory: For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	0.2	20ng/ml
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PMP21 may be used as a standard for ELISA applications with AAM32
Western Blotting: PMP21 may be used as a positive control for Western blotting applications with AAM32

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Mouse Interleukin-10	AAM32	E FN WB	0.1 mg	Log in		Get Quote

Product Specific References

References for IL-10

Gelderblom, H. et al. (2007) Role of interleukin 10 during persistent infection with the relapsing fever Spirochete Borrelia turicatae.
Am J Pathol. 170: 251-62.

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