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IFN Gamma

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Recombinant Mouse Interferon Gamma thumbnail image 1
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Published customer image:
Murine macrophages cultured in the presence of mouse interferon-γ (PMP43A) or mouse interleukin-4 (PMP81 ) to generate an M1 and M2 phenotype respectively. cells were sublequently exposed to bacteria as indicated to visualise phagocytosis.
Image caption:
3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages.
(A,B,C) Maximum intensity projection and (D,E,F) the corresponding slice view 3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages (60:1 BMR, 1 hour exposure time), respectively, on the Deltavision OMX Structured Illumination Microscope V4 Blaze (Applied Precision, WA, USA). P. gingivalis W50 were labelled with AF488 (green) while the nuclei and actin filaments of the macrophages were stained with DAPI (blue) and Phalloidin-TRITC (red) respectively. The slice images were obtained from a section with defined size (between 10–50 z-stack) to confirm phagocytosis of P. gingivalis W50 by naive, M1 and M2 macrophages.

From: Lam RS, O'Brien-Simpson NM, Holden JA, Lenzo JC, Fong SB, Reynolds EC (2016)
Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS ONE 11(7): e0158629.

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Recombinant Mouse Interferon Gamma

Product Type
Recombinant Protein
Specificity
IFN Gamma

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Product Code Applications Pack Size List Price Your Price Qty
PMP43A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 20 µg
List Price Your Price

Recombinant mouse interferon gamma is an E. coli-derived recombinant protein corresponding to full-length mature murine interferon gamma, a regulatory cytokine, which is produced by activated natural killer cells and certain T-lymphocytes.

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20ul water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Escherichia coli
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
>5 x 106 units/mg as determined by ability to inhibit the proliferation of murine WEHI-279 cells.
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml after reconstitution
Protein Molecular Weight
15.6kD (134 amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 1.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
PMP43A may be used as a standard in ELISA applications with AAM29
Western Blotting
PMP43A may be used as a positive control in Western blotting applications with AAM29

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Mouse Interferon Gamma AAM29 E FN WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit anti Mouse Interferon Gamma

References for IFN Gamma

  1. Zhang, H. et al. (2011) Matrix metalloproteinase-9 and stromal cell-derived factor-1 act synergistically to support migration of blood-borne monocytes into the injured spinal cord.
    J Neurosci. 31: 15894-903.
  2. Wimmer, N. et al. (2012) Lymphotoxin β receptor activation on macrophages induces cross-tolerance to TLR4 and TLR9 ligands.
    J Immunol. 188 (7): 3426-33.
  3. Lam RS et al. (2016) Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
    PLoS One. 11 (7): e0158629.
  4. Lestinova, T. et al. (2015) Phlebotomus papatasi exposure cross-protects mice against Leishmania major co-inoculated with Phlebotomus duboscqi salivary gland homogenate.
    Acta Trop. 144: 9-18.

Synonyms
Interferon Gamma
UniProt
P01580
Entrez Gene
Ifng
GO Terms
GO:0005622 intracellular
GO:0001781 neutrophil apoptosis
GO:0042742 defense response to bacterium
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006925 inflammatory cell apoptosis
GO:0006955 immune response
GO:0019882 antigen processing and presentation
GO:0030593 neutrophil chemotaxis
GO:0030968 endoplasmic reticulum unfolded protein response
GO:0031642 negative regulation of myelination
GO:0032735 positive regulation of interleukin-12 production
GO:0044130 negative regulation of growth of symbiont in host
GO:0042102 positive regulation of T cell proliferation
GO:0042832 defense response to protozoan
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045084 positive regulation of interleukin-12 biosynthetic process
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050718 positive regulation of interleukin-1 beta secretion

PMP43A

155897 160721 162645 165010

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