Recombinant mouse interferon gamma is an E. coli-derived recombinant protein corresponding to full-length mature murine interferon gamma, a regulatory cytokine, which is produced by activated natural killer cells and certain T-lymphocytes.
- Target Species
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitute with 20ul water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Recombinant protein expressed in Escherichia coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- >5 x 106 units/mg as determined by ability to inhibit the proliferation of murine WEHI-279 cells.
- >98% by SDS PAGE and HPLC
- Approx. Protein Concentrations
- Total protein concentration 1.0mg/ml after reconstitution
- Protein Molecular Weight
- 15.6kD (134 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
defense response to bacterium
response to virus
interferon-gamma receptor binding
inflammatory cell apoptosis
antigen processing and presentation
endoplasmic reticulum unfolded protein response
negative regulation of myelination
positive regulation of interleukin-12 production
negative regulation of growth of symbiont in host
positive regulation of T cell proliferation
defense response to protozoan
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-dependent
positive regulation of isotype switching to IgG isotypes
positive regulation of interleukin-1 beta secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Gamma
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
- PMP43A may be used as a standard in ELISA applications with AAM29
- Western Blotting
- PMP43A may be used as a positive control in Western blotting applications with AAM29
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Useful Reagents Available
Product Specific References
References for IFN Gamma
Zhang, H. et al. (2011) Matrix metalloproteinase-9 and stromal cell-derived factor-1 act synergistically to support migration of blood-borne monocytes into the injured spinal cord.
J Neurosci. 31: 15894-903.
Wimmer, N. et al. (2012) Lymphotoxin β receptor activation on macrophages induces cross-tolerance to TLR4 and TLR9 ligands.
J Immunol. 188 (7): 3426-33.
Lam RS et al. (2016) Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS One. 11 (7): e0158629.
Lestinova, T. et al. (2015) Phlebotomus papatasi exposure cross-protects mice against Leishmania major co-inoculated with Phlebotomus duboscqi salivary gland homogenate.
Acta Trop. 144: 9-18.
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