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Collagen I

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Native Collagen I (Tail Tendon) thumbnail image 1
Native Collagen I (Tail Tendon) gallery image 1
Published customer image:
Purified Mouse collagen I (2150-1425) used to coat culture dishes for the propagation of reactive astrocytes (RAs).
Image caption:
(h) Example images depicting immunocytological analysis of RAs on noncoated or Col I–coated dishes for GFAP (green) and N-cadherin (red) with Hoechst staining (blue). Scale bar, 50μm.

From: Hara M, Kobayakawa K, Ohkawa Y, Kumamaru H, Yokota K, Saito T, Kijima K, Yoshizaki S, Harimaya K, Nakashima Y, Okada S.
Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury.
Nat Med. 2017 Jul;23(7):818-28.
doi: 10.1038/nm.4354.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Native Collagen I (Tail Tendon)

Product Type
Purified Protein
Specificity
Collagen I
Region
(TAIL TENDON)

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Product Code Applications Pack Size List Price Your Price Qty
2150-1425
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SDS Safety Datasheet SDS
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Native Murine collagen I is purified Mouse collagen I from tail tendon. Thermal denaturation converts the collagen to gelatin.

Impurities:
Mouse collagen type III 10%
Mouse collagen (other types) <1%
Non-collagenous proteins <0.5%

Target Species
Mouse
Product Form
Purified Protein - liquid
Preparation
Collagens were extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Buffer Solution
0.5M acetic acid
Preservative Stabilisers
None present
Purity
90%< by SDS PAGE (cross linked collagen type I dimers and trimers represent ~10%)
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
~300 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Collagen I

  1. Sebinger, D.D. et al. (2013) ECM modulated early kidney development in embryonic organ culture.
    Biomaterials. 34 (28): 6670-82.
  2. Takahashi, S. et al. (2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.
    FEBS Lett. 589 (7): 829-35.
  3. Hara, M. et al. (2017) Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury.
    Nat Med. 23 (7): 818-28.
  4. Tamaru, T. et al. (2023) Glial scar survives until the chronic phase by recruiting scar-forming astrocytes after spinal cord injury.
    Exp Neurol. 359: 114264.

Further Reading

  1. Rhodes, R.K. & Miller, E.J. (1978) Physicochemical characterization and molecular organization of the collagen A and B chains.
    Biochemistry. 17 (17): 3442-8.

UniProt
P11087
Q01149
Entrez Gene
Col1a1
Col1a2
GO Terms
GO:0001568 blood vessel development
GO:0001649 osteoblast differentiation
GO:0001957 intramembranous ossification
GO:0001958 endochondral ossification
GO:0005201 extracellular matrix structural constituent
GO:0005584 collagen type I
GO:0005737 cytoplasm
GO:0010812 negative regulation of cell-substrate adhesion
GO:0015031 protein transport
GO:0060325 face morphogenesis
GO:0060346 bone trabecula formation
GO:0060351 cartilage development involved in endochondral bone morphogenesis
GO:0070208 protein heterotrimerization
GO:0046332 SMAD binding

2150-1425

148861 152452 166533

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