Collagen I/III

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Native Mouse Collagen I/III

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-2506 E 10 mg
Murine collagen type I 45%
Murine collagen type III 45%
Murine collagen type IV 10%
Murine collagen type V <1%
Non-collagenous proteins <0.5%
M[a1(I)1a2(I)2]. Native triple helix.

Product Details

Target Species
Mouse
Product Form
Purified protein from murine tail tendons - lyophilised
Reconstitution
Use 0.05-0.5 M acetic acid, pH 2.5 at 4ÂșC. Dissolved collagen retains immunologic properties of native collagen types I+III. Thermal denaturation converts dissolved collagen to gelatin.
Preparation
Pepsin treatment, acetic acid extraction, serial salt precipitations.
Buffer Solution
Essentially salt free
Preservative Stabilisers
None present.
Purity
Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement and ability to form microfibrils.
Molecular Weight
MW: 300 kDa

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11087 Related reagents
Q01149 Related reagents
P08121 Related reagents
Entrez Gene
Col3a1 Related reagents
Col1a1 Related reagents
Col1a2 Related reagents
GO Terms
GO:0001568 blood vessel development
GO:0005586 collagen type III
GO:0030199 collagen fibril organization
GO:0046332 SMAD binding
GO:0048565 digestive tract development
GO:0001649 osteoblast differentiation
GO:0001957 intramembranous ossification
GO:0001958 endochondral ossification
GO:0005201 extracellular matrix structural constituent
GO:0005584 collagen type I
GO:0005737 cytoplasm
GO:0010812 negative regulation of cell-substrate adhesion
GO:0015031 protein transport
GO:0060325 face morphogenesis
GO:0060346 bone trabecula formation
GO:0060351 cartilage development involved in endochondral bone morphogenesis
GO:0070208 protein heterotrimerization
Regulatory
For research purposes only

Applications of Collagen I/III

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Collagen I/III

  1. Rhodes, R. K. & Miller, E. J. (1978) Physicochemical characterization and molecular organization of the collagen A and B chains
    Biochemistry 17: 3442 - 3448