Collagen I/III
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Murine collagen type I 45%
Murine collagen type III 45%
Murine collagen type IV 10%
Murine collagen type V <1%
Non-collagenous proteins <0.5%
M[a1(I)1a2(I)2]. Native triple helix.
Murine collagen type III 45%
Murine collagen type IV 10%
Murine collagen type V <1%
Non-collagenous proteins <0.5%
M[a1(I)1a2(I)2]. Native triple helix.
Product Details
- Target Species
- Mouse
- Product Form
- Purified protein from murine tail tendons - lyophilized
- Reconstitution
- Use 0.05-0.5 M acetic acid, pH 2.5 at 4ºC. Dissolved collagen retains immunologic properties of native collagen types I+III. Thermal denaturation converts dissolved collagen to gelatin.
- Preparation
- Pepsin treatment, acetic acid extraction, serial salt precipitations
- Buffer Solution
- Essentially salt free
- Preservative Stabilisers
- None present
- Purity
- Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement and ability to form microfibrils.
- Protein Molecular Weight
- 300 kDa
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P11087
- Q01149
- P08121
- Entrez Gene
- Col3a1
- Col1a1
- Col1a2
- GO Terms
- GO:0001568 blood vessel development
- GO:0005586 collagen type III
- GO:0030199 collagen fibril organization
- GO:0046332 SMAD binding
- GO:0048565 digestive tract development
- GO:0001649 osteoblast differentiation
- GO:0001957 intramembranous ossification
- GO:0001958 endochondral ossification
- GO:0005201 extracellular matrix structural constituent
- GO:0005584 collagen type I
- GO:0005737 cytoplasm
- GO:0010812 negative regulation of cell-substrate adhesion
- GO:0015031 protein transport
- GO:0060325 face morphogenesis
- GO:0060346 bone trabecula formation
- GO:0060351 cartilage development involved in endochondral bone morphogenesis
- GO:0070208 protein heterotrimerization
- Regulatory
- For research purposes only
Applications of Collagen I/III
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Collagen I/III
-
Rhodes, R.K. & Miller, E.J. (1978) Physicochemical characterization and molecular organization of the collagen A and B chains.
Biochemistry. 17 (17): 3442-8.
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