VEGF

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Recombinant Human VEGF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP091 E FN WB 10 µg
VEGF is a homodimeric protein produced by various vascularized tissues. Activities include stimulation of endothelial cell growth, angiogenesis and capillary permeability.

Product Details

Target Species
Human
Product Form
Purified recombinant protein expressed in E. coli - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml
Protein Molecular Weight
38kD (2 x 165 Amino acid sequence)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P15692 Related reagents
Entrez Gene
VEGFA Related reagents
Regulatory
For research purposes only

Applications of VEGF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
PHP091 may be used in ELISA applications with either AHP831 or AHP831B.
Western Blotting
PHP091 may be used under reducing or non-reducing western blotting conditions with either AHP831 or AHP831B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human VEGF AHP831 0.1 mg E FN P* WB
Goat anti Human VEGF:Biotin AHP831B 50 µg E WB

Product Specific References

References for VEGF

  1. Easton, C.D. et al. (2014) Application of layer-by-layer coatings to tissue scaffolds – development of an angiogenic biomaterial.
    Journal of Materials Chemistry B. 2, 5558-68