VEGF
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VEGF is a homodimeric protein produced by various vascularized tissues. Activities include stimulation of endothelial cell growth, angiogenesis and capillary permeability.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilised
- Reconstitution
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Purity
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 38kD (2 x 165 Amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
Applications of VEGF
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Functional Assays | 1 | 100ng/ml | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human VEGF | AHP831 | E FN P* WB | 0.1 mg |
|
|
| Goat anti Human VEGF:Biotin | AHP831B | E WB | 50 µg |
|
Product Specific References
References for VEGF
-
Easton, C.D. et al. (2014) Application of layer-by-layer coatings to tissue scaffolds – development of an angiogenic biomaterial.
Journal of Materials Chemistry B. 2, 5558-68
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