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TNF Alpha

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Recombinant Human TNF Alpha thumbnail image 1 Recombinant Human TNF Alpha thumbnail image 2
Recombinant Human TNF Alpha gallery image 1
Published customer image:
Recombinant human TNF-α (PHP051) used to treat RSFLs prior to evaluation of VE-CDH by western blotting.
Image caption:
Protein expression of VE-cadherin species in RSFLs under the simulation.
(c) Immunoblot of intact and cytoplasmic fragment of VE-CDH in RSFL4 after treatment with cytokines or under hypoxia. The cells were treated with TNF-a in the absence or presence of VEGF165 and under hypoxic conditions of 1% O2 or deferoxamine treatment (DFX). Ctrl, control without treatment. (d) Protein band densities of VE-CDH (125 kDa and 35 kDa) in RSFL4 compared with Ctrl RSFL4.
From: Yamazaki N, et al.
VE-cadherin-dependent vasculogenic mimicry-like tube formation in rheumatoid arthritic synovium.
Research Square 10 October 2022.
doi: 10.21203/rs.3.rs-2127147/v1
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Human TNF Alpha gallery image 2
Published customer image:
Recombinant human TNF-α (PHP051) used to treat RSFLs prior to evaluation of VE-CDH by western blotting.
Image caption:
Expression of VEGF isoforms and VEGF receptors in RSFLs and changes in their expression under various conditions.
(c) Expression of VEGFRs including NRP1, VEGFR1 and VEGFR2 in RSFLs under treatment with or without TNF-α or hypoxia (1% O2). (d) Protein band densities of NRP1 and VEGFR1 compared to control cells (n = 4).
From: Yamazaki N, et al.
VE-cadherin-dependent vasculogenic mimicry-like tube formation in rheumatoid arthritic synovium.
Research Square 10 October 2022.
doi: 10.21203/rs.3.rs-2127147/v1
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Recombinant Human TNF Alpha

Product Type
Recombinant Protein
Specificity
TNF Alpha

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Product Code Applications Pack Size List Price Your Price Qty
PHP051A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 10 µg loader
List Price Your Price
loader
PHP051
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 50 µg loader
List Price Your Price
loader

Recombinant Human tumor necrosis factor alpha is an E. coli-derived recombinant protein corresponding to the C-terminal domain of human tumour necrosis factor alpha (TNFa), a pro-inflammatory cytokine with cytotoxic effects on various tumour cells.

Target Species
Human
Product Form
Purified recombinant cytokine - lyophilized
Reconstitution
PHP051A
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
PHP051
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Source
E.coli
Buffer Solution
PHP051A: Phosphate buffered saline
PHP051: Sodium Phosphate
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
PHP051A: PHP051A has a specific activity of >2 x 107 units/mg as determined by cytolysis of L929 cells in the presence of Actinomycin D.
PHP051: The activity of this protein has been tested on murine L929 cells.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight
PHP051A: 17.4 kDa based on 157 amino acid sequence
PHP051: 17.4kD (157 Amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.05ng/ml Pack Size: 10 µg
0.05 Pack Size: 50 µg		 20ng/ml Pack Size: 10 µg
20.0ng/ml Pack Size: 50 µg
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
PHP051 may be used as a standard in a sandwich ELISA with AHP1212 as the capture reagent and AHP1212B as the detection reagent.
Western Blotting
PHP051 may be used as a positive control in western blotting for either AHP1212 or AHP1212B.

References for TNF Alpha

  1. Cernuda-morollón, E. et al. (2001) 15-Deoxy-Delta 12,14-prostaglandin J2 inhibition of NF-kappaB-DNA binding through covalent modification of the p50 subunit.
    J Biol Chem. 276 (38): 35530-6.
  2. Dernfalk, J. et al. (2004) Commercially available antibodies to human tumour necrosis factor-alpha tested for cross-reactivity with ovine and bovine tumour necrosis factor-alpha using flow cytometric assays.
    Acta Vet Scand. 45: 99-107.
  3. Taubert, A. et al. (2007) Eimeria bovis infection enhances adhesion of peripheral blood mononuclear cells to and their transmigration through an infected bovine endothelial cell monolayer in vitro.
    Parasitol Res. 101: 591-8.
  4. Taubert, A. & Hermosilla, C. (2008) Bovine recombinant IFNgamma induces endothelial cell gene transcription of immunoregulatory molecules and upregulates PMN and PBMC adhesion on bovine endothelial cells.
    Vet Res Commun. 32: 35-47.
  5. Gerber, P.A. et al. (2016) Mechanisms of skin aging induced by EGFR inhibitors.
    Support Care Cancer. 24 (10): 4241-8.
  6. Hara, H. et al. (2021) Stable expression of the human thrombomodulin transgene in pig endothelial cells is associated with a reduction in the inflammatory response.
    Cytokine. 148: 155580.
  7. Yamazaki, N. et al. (2022) VE-cadherin-dependent vasculogenic mimicry-like tube formation in rheumatoid arthritic synovium,
    Res Sq. 10 Oct [Epub ahead of print].
  8. Kislat, A. et al. (2023) The Endogenous Dual Retinoid Receptor Agonist Alitretinoin Exhibits Immunoregulatory Functions on Antigen-Presenting Cells.
    Int J Mol Sci. 24 (11) :9654.
    9. View The Latest Product References

ELISA

Functional Assays

UniProt
P01375
Entrez Gene
TNF
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0000185 activation of MAPKKK activity
GO:0000187 activation of MAPK activity
GO:0001891 phagocytic cup
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0009615 response to virus
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0006927 transformed cell apoptosis
GO:0050796 regulation of insulin secretion
GO:0042802 identical protein binding
GO:0009651 response to salt stress
GO:0009897 external side of plasma membrane
GO:0010843 promoter binding
GO:0010888 negative regulation of lipid storage
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030730 sequestering of triglyceride
GO:0031334 positive regulation of protein complex assembly
GO:0031622 positive regulation of fever generation
GO:0032582 negative regulation of gene-specific transcription
GO:0032715 negative regulation of interleukin-6 production
GO:0032722 positive regulation of chemokine production
GO:0032800 receptor biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034116 positive regulation of heterotypic cell-cell adhesion
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043193 positive regulation of gene-specific transcription
GO:0043243 positive regulation of protein complex disassembly
GO:0045071 negative regulation of viral genome replication
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045121 membrane raft
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045672 positive regulation of osteoclast differentiation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050715 positive regulation of cytokine secretion
GO:0050901 leukocyte tethering or rolling
GO:0050995 negative regulation of lipid catabolic process
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0055037 recycling endosome
GO:0060555 induction of necroptosis by extracellular signals
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0061048 negative regulation of branching involved in lung morphogenesis
GO:0070265 necrotic cell death
GO:0070374 positive regulation of ERK1 and ERK2 cascade

PHP051A

154805 155576 160137 161388 164893 167000 168657

PHP051

149599 156688 160284 166163 168492

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