IL-25
Recombinant Human interleukin-25 (PHP300) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands were visualized using Coomassie stains. The purified recombinant interleukin-25 runs at a higher molecular weight than the theoretical molecular weight. This is likely due to the presence of posttranslational modifications as a result of the expression of the recombinant interleukin-25 in the mammalian cell line HEK293.
Recombinant Human Interleukin-25
- Product Type
- Recombinant Protein
- Specificity
- IL-25
| Recombinant Human interleukin-25 Interleukin-25 (IL-25) also known as IL17E is a member of the IL-17 family of cytokines and is expressed by various immune cells including mast cells, eosinophils, basophils and T helper type 2 cells. It is also expressed in non-hematopoietic cells such as epithelial and endothelial cells (Yao et al. 2016). As a pleiotropic cytokine, it stimulates stromal, innate immune and adaptive immune cells to regulate Th2 type inflammation (Monin & Gaffen 2018). IL-25 exerts its biological function through its receptor IL-17RB, although there is some evidence that it may also bind to the receptor IL-17RA (Yao et al. 2016). Through this interaction, IL-25 is able to induce expression of IL-4, IL-5, IL-13 and TSLP (McGeachy et al. 2019). IL-25 is upregulated in asthmatic conditions and can induce typical eosinophil inflammation and airway hyperresponsiveness (Yao et al. 2016). IL-25 levels are also associated with the severity of chronic allergic reactions (Monin & Gaffen 2018). This recombinant Human interleukin-25 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-25 antibody, clone A09 -3D10 (MCA6248GA) as a capture antibody and biotinylated Mouse anti Human interleukin-25 antibody, clone A14 -3D2 (MCA6249B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex. - Preparation
- Recombinant protein expressed in HEK293 cells and purified by ion exchange chromatography
- Source
- HEK-293
- Buffer Solution
- 50 mM Tris-HCl, pH 8.0, 0.15 M NaCl
- Preservative Stabilisers
- 1.0% Trehalose
- Purity
- ≥90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
- Approx. Protein Concentrations
- 100 μg/ml after reconstitution
- Protein Molecular Weight
- 16.7 kDa
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Capture Antibody:
MCA6248GA
ELISA Matched Pair-Detection Antibody:
MCA6249B
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human Interleukin-25 | MCA6248GA | E | 0.1 mg |
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| Description | Mouse anti Human Interleukin-25 | ||||||
| Mouse anti Human Interleukin-25:Biotin | MCA6249B | E | 0.1 mg |
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| Description | Mouse anti Human Interleukin-25:Biotin | ||||||
- Synonyms
- Interleukin-17E
- UniProt
- Q9H293
- Entrez Gene
- IL25
- GO Terms
- GO:0016020 membrane
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0030380 interleukin-17E receptor binding
PHP300
100005641
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