IL-25 antibody | A14-3D2
Mouse anti Human Interleukin-25:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- A14-3D2
- Isotype
- IgG1
- Specificity
- IL-25
| Mouse anti Human interleukin-25, clone A14-3D2, recognizes interleukin-25 (IL-25) also known as IL17E. IL-25 is a member of the IL-17 family of cytokines and is expressed by various immune cells including mast cells, eosinophils, basophils and T helper type 2 cells. It is also expressed in non-hematopoietic cells such as epithelial and endothelial cells (Yao et al. 2016). As a pleiotropic cytokine, it stimulates stromal, innate immune and adaptive immune cells to regulate Th2 type inflammation (Monin & Gaffen 2018). IL-25 exerts its biological function through its receptor IL-17RB, although there is some evidence that it may also bind to the receptor IL-17RA (Yao et al. 2016). Through this interaction, IL-25 is able to induce expression of IL-4, IL-5, IL-13 and TSLP (McGeachy et al. 2019). IL-25 is upregulated in asthmatic conditions and can induce typical eosinophil inflammation and airway hyperresponsiveness (Yao et al. 2016). IL-25 levels are also associated with the severity of chronic allergic reactions (Monin & Gaffen 2018). The biotinylated Mouse anti Human interleukin-25 antibody, clone A14 -3D2 (MCA6249B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-25 antibody, clone A09 -3D10 (MCA6248GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- E. coli derived recombinant human full length interleukin-25 (Tyr33-Gly177)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Capture Antibody:
MCA6248GA
ELISA Antigen-Positive Control:
PHP300
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human Interleukin-25 | MCA6248GA | E | 0.1 mg |
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| Description | Mouse anti Human Interleukin-25 | ||||||
| Recombinant Human Interleukin-25 | PHP300 | E | 10 µg |
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| Description | Recombinant Human Interleukin-25 | ||||||
- UniProt
- Q9H293
- Entrez Gene
- IL25
- GO Terms
- GO:0016020 membrane
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0030380 interleukin-17E receptor binding
MCA6249B
100005147
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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