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IL-25 antibody | A14-3D2

Mouse anti Human Interleukin-25:Biotin

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Mouse anti Human interleukin-25, clone A14-3D2, recognizes interleukin-25 (IL-25) also known as IL17E. IL-25 is a member of the IL-17 family of cytokines and is expressed by various immune cells including mast cells, eosinophils, basophils and T helper type 2 cells. It is also expressed in non-hematopoietic cells such as epithelial and endothelial cells (Yao et al. 2016). As a pleiotropic cytokine, it stimulates stromal, innate immune and adaptive immune cells to regulate Th2 type inflammation (Monin & Gaffen 2018). IL-25 exerts its biological function through its receptor IL-17RB, although there is some evidence that it may also bind to the receptor IL-17RA (Yao et al. 2016). Through this interaction, IL-25 is able to induce expression of IL-4, IL-5, IL-13 and TSLP (McGeachy et al. 2019).

IL-25 is upregulated in asthmatic conditions and can induce typical eosinophil inflammation and airway hyperresponsiveness (Yao et al. 2016). IL-25 levels are also associated with the severity of chronic allergic reactions (Monin & Gaffen 2018).

The biotinylated Mouse anti Human interleukin-25 antibody, clone A14 -3D2 (MCA6249B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-25 antibody, clone A09 -3D10 (MCA6248GA) as the capture antibody.

Target Species
Product Form
Purified IgG conjugated to Biotin - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
E. coli derived recombinant human full length interleukin-25 (Tyr33-Gly177)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6248GA ELISA Antigen-Positive Control: PHP300
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Interleukin-25 MCA6248GA E 0.1 mg loader
List Price Your Price
Description Mouse anti Human Interleukin-25
Recombinant Human Interleukin-25 PHP300 E 10 µg loader
List Price Your Price
Description Recombinant Human Interleukin-25

Entrez Gene
GO Terms
GO:0016020 membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0030380 interleukin-17E receptor binding



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