IL-12

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Recombinant Human Interleukin-12

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PHP100	E FN WB			10 µg

Summary

Recombinant human Interleukin-12 (IL-12) produced in CHO cells is a ~75 kDa glycoprotein comprised of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits.

IL-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes, and actions in the development of Th1 CD4 +ve cells and CD8 +ve cytotoxic T cells. IL-12 also promotes activity and division of NK cells and promotes formation of TH1 cells producing &gamma-interferon.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilised
Reconstitution: Reconstitute with 50ul sterile PBS. For extended storage, the addition of 0.1% bovine serum albumin is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation: Purified recombinant Human Interleukin-12 expressed in Chinese Hamster Ovary (CHO) cells.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.2 mg/ml after reconstitution
Protein Molecular Weight: 75kDa (503 Amino acid sequence)
Endotoxin Level: <0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

More Information

UniProt: P29460 Related reagents; P29459 Related reagents
Entrez Gene: IL12A Related reagents; IL12B Related reagents
GO Terms: GO:0001916 positive regulation of T cell mediated cytotoxicity; GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; GO:0009615 response to virus; GO:0005125 cytokine activity; GO:0005143 interleukin-12 receptor binding; GO:0006955 immune response; GO:0007050 cell cycle arrest; GO:0008083 growth factor activity; GO:0050830 defense response to Gram-positive bacterium; GO:0010224 response to UV-B; GO:0016477 cell migration; GO:0032496 response to lipopolysaccharide; GO:0032700 negative regulation of interleukin-17 production; GO:0032729 positive regulation of interferon-gamma production; GO:0032816 positive regulation of natural killer cell activation; GO:0034393 positive regulation of smooth muscle cell apoptosis; GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein; GO:0043514 interleukin-12 complex; GO:0045513 interleukin-27 binding; GO:0045785 positive regulation of cell adhesion; GO:0046982 protein heterodimerization activity; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0051135 positive regulation of NK T cell activation; GO:0002230 positive regulation of defense response to virus by host; GO:0002827 positive regulation of T-helper 1 type immune response; GO:0016020 membrane; GO:0004896 cytokine receptor activity; GO:0050829 defense response to Gram-negative bacterium; GO:0010535 positive regulation of activation of JAK2 kinase activity; GO:0019953 sexual reproduction; GO:0032693 negative regulation of interleukin-10 production; GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production; GO:0032733 positive regulation of interleukin-10 production; GO:0032735 positive regulation of interleukin-12 production; GO:0032740 positive regulation of interleukin-17 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0032819 positive regulation of natural killer cell proliferation; GO:0034105 positive regulation of tissue remodeling; GO:0042088 T-helper 1 type immune response; GO:0042093 T-helper cell differentiation; GO:0042095 interferon-gamma biosynthetic process; GO:0042104 positive regulation of activated T cell proliferation; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein; GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0043382 positive regulation of memory T cell differentiation; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045672 positive regulation of osteoclast differentiation; GO:0050729 positive regulation of inflammatory response; GO:0051142 positive regulation of NK T cell proliferation; GO:0070743 interleukin-23 complex
Regulatory: For research purposes only

Applications of IL-12

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4 ng/well
Functional Assays	0.1	10 ng/ml
Western Blotting	1.5	3.0 ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.

ELISA: Recombinant human IL-12 may be used as a standard for ELISA applications with either anti-human IL-12:purified (AHP779) or anti-human IL-12:biotin conjugate (AHP779B).
Western Blotting: Recombinant human IL-12 may be used as thepostivie control for wester blotting applications with either anti-human IL-12:purified (AHP779) or anti-human IL-12:biotin conjugate (AHP779B).

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