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IL-12

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Recombinant Human Interleukin-12

Product Type
Recombinant Protein
Specificity
IL-12

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Recombinant human Interleukin-12 (IL-12) produced in CHO cells is a ~75 kDa glycoprotein comprised of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits.

IL-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes, and actions in the development of Th1 CD4 +ve cells and CD8 +ve cytotoxic T cells. IL-12 also promotes activity and division of NK cells and promotes formation of TH1 cells producing &gamma-interferon.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 50ul sterile PBS. For extended storage, the addition of 0.1% bovine serum albumin is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Purified recombinant Human Interleukin-12 expressed in Chinese Hamster Ovary (CHO) cells.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.2 mg/ml after reconstitution
Protein Molecular Weight
75kDa (503 Amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4 ng/well
Functional Assays 0.1 10 ng/ml
Western Blotting 1.5 3.0 ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant human IL-12 may be used as a standard for ELISA applications with either anti-human IL-12:purified (AHP779) or anti-human IL-12:biotin conjugate (AHP779B).
Western Blotting
Recombinant human IL-12 may be used as the positive control for western blotting applications with either anti-human IL-12:purified (AHP779) or anti-human IL-12:biotin conjugate (AHP779B).

UniProt
P29460
P29459
Entrez Gene
IL12A
IL12B
GO Terms
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0010224 response to UV-B
GO:0016477 cell migration
GO:0032496 response to lipopolysaccharide
GO:0032700 negative regulation of interleukin-17 production
GO:0032729 positive regulation of interferon-gamma production
GO:0032816 positive regulation of natural killer cell activation
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein
GO:0043514 interleukin-12 complex
GO:0045513 interleukin-27 binding
GO:0045785 positive regulation of cell adhesion
GO:0046982 protein heterodimerization activity
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051135 positive regulation of NK T cell activation
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0050829 defense response to Gram-negative bacterium
GO:0010535 positive regulation of activation of JAK2 kinase activity
GO:0019953 sexual reproduction
GO:0032693 negative regulation of interleukin-10 production
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032740 positive regulation of interleukin-17 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0032819 positive regulation of natural killer cell proliferation
GO:0034105 positive regulation of tissue remodeling
GO:0042088 T-helper 1 type immune response
GO:0042093 T-helper cell differentiation
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043382 positive regulation of memory T cell differentiation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0050729 positive regulation of inflammatory response
GO:0051142 positive regulation of NK T cell proliferation
GO:0070743 interleukin-23 complex
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