IL-12 antibody
Goat anti Human Interleukin-12
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-12
Goat anti Human Interleukin-12 antibody, recognizes human IL-12. IL-12 is a pleiotropic 75kDa heterodimeric glycoprotein, consisting of disulfide-linked IL-12A (p35) and IL-12B (p40) subunits, which signals through the high affinity IL-12 receptor. IL-12 is produced primarily by antigen presenting cells and was originally called cytotoxic lymphocyte maturation factor (CLMF), identified by its ability to induce cytotoxic effector cells, in synergy with IL-2. IL-12 is an important factor in the T helper cell (Th1) immune response, promotes the development and activity of activated T cells and natural killer cells (NK), induces IFN-gamma production, and enhances resistance against invading pathogens. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. N.B For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-12 (PHP100)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Functional Assays | 0.012 | 0.02ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
- ELISA
- Anti human IL-12:purified may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with anti human IL-12:biotin conjugate (AHP779B) as the detection reagent and recombinant human IL-12 (PHP100) as the standard.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Rabbit anti Goat IgG (Fc):HRP |
References for IL-12 antibody
-
Munari, F. et al. (2014) Cytokine BAFF released by Helicobacter pylori-infected macrophages triggers the Th17 response in human chronic gastritis.
J Immunol. 193 (11): 5584-94.
Further Reading
-
Gubler, U. et al. (1991) Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.
Proc Natl Acad Sci U S A. 88 (10): 4143-7. -
Rossol, S. et al. (1997) Interleukin-12 induction of Th1 cytokines is important for viral clearance in chronic hepatitis B.
J Clin Invest. 99 (12): 3025-33.
- RRID
- AB_2123618
- UniProt
- P29459
- P29460
- Entrez Gene
- IL12A
- IL12B
- GO Terms
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005143 interleukin-12 receptor binding
- GO:0006955 immune response
- GO:0007050 cell cycle arrest
- GO:0008083 growth factor activity
- GO:0050830 defense response to Gram-positive bacterium
- View More GO Terms
- GO:0010224 response to UV-B
- GO:0016477 cell migration
- GO:0032496 response to lipopolysaccharide
- GO:0032700 negative regulation of interleukin-17 production
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032816 positive regulation of natural killer cell activation
- GO:0034393 positive regulation of smooth muscle cell apoptosis
- GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein
- GO:0043514 interleukin-12 complex
- GO:0045513 interleukin-27 binding
- GO:0045785 positive regulation of cell adhesion
- GO:0046982 protein heterodimerization activity
- GO:0048662 negative regulation of smooth muscle cell proliferation
- GO:0051135 positive regulation of NK T cell activation
- GO:0002230 positive regulation of defense response to virus by host
- GO:0002827 positive regulation of T-helper 1 type immune response
- GO:0016020 membrane
- GO:0004896 cytokine receptor activity
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010535 positive regulation of activation of JAK2 kinase activity
- GO:0019953 sexual reproduction
- GO:0032693 negative regulation of interleukin-10 production
- GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032740 positive regulation of interleukin-17 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0032819 positive regulation of natural killer cell proliferation
- GO:0034105 positive regulation of tissue remodeling
- GO:0042088 T-helper 1 type immune response
- GO:0042093 T-helper cell differentiation
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043382 positive regulation of memory T cell differentiation
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0050729 positive regulation of inflammatory response
- GO:0051142 positive regulation of NK T cell proliferation
- GO:0070743 interleukin-23 complex
AHP779
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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