Human IgG1 | AbD18705_hIgG1
Recombinant Human IgG1 Kappa was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands were visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated. The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG1 Kappa. The larger peak corresponds to monomeric, fully assembled AbD18705_hIgG1. The smaller shoulder most likely contains dimers and aggregates
Recombinant Human IgG1 Kappa, clone AbD18705_hIgG1 is a recombinant human IgG1 antibody with a kappa light chain and a heavy chain belonging to the G1m(f) allotype, previously designated G1m3 (GenBank AAT49050).
Recombinant Human IgG1 Kappa, clone AbD18705_hIgG1 is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG1 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.
Recombinant Human IgG1 Kappa, clone AbD18705_hIgG1 forms part of a range of human recombinant immunoglobulins containing the kappa light chain. A similar range of recombinant human immunoglobulins containing the lambda light chain is also available from Bio-Rad.
Recombinant Human IgG1 Kappa, clone AbD18705_hIgG1 is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG1 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.
Recombinant Human IgG1 Kappa, clone AbD18705_hIgG1 forms part of a range of human recombinant immunoglobulins containing the kappa light chain. A similar range of recombinant human immunoglobulins containing the lambda light chain is also available from Bio-Rad.
Product Details
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line -This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Pack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01834
- P01857
- Entrez Gene
- IGKC
- IGHG1
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Human IgG1
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Human IgG1 Lambda Allotype G1m3 | HCA049 | E | 0.1 mg |
|
