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IgA

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Sandwich ELISA analysis of human IgA binding using Mouse anti Human IgA MCA476G) as a capture reagent and biotinylated Mouse anti Human IgA (5104-3104) as a detection reagent with purified human IgA (PHP133) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions.

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Purified Human Secretory IgA

Product Type
Purified Protein
Specificity
IgA
Region
(SECRETORY)

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Product Code Applications Pack Size List Price Your Price Qty
PHP133
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader

Purified Human Secretory IgA is derived from human colostrum and is predominately in dimeric form, the monomers being linked by immunoglobulin J chain.

Target Species
Human
Product Form
Purified IgA - liquid
Preparation
Purified human secretory IgA prepared by size-exclusion and ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgA

  1. Bidgood, S.R. et al. (2014) Translocalized IgA mediates neutralization and stimulates innate immunity inside infected cells.
    Proc Natl Acad Sci U S A. 111 (37): 13463-8.
  2. Herrera, D. et al. (2013) Rotavirus specific plasma secretory immunoglobulin in children with acute gastroenteritis and children vaccinated with an attenuated human rotavirus vaccine.
    Hum Vaccin Immunother. 9 (11): 2409-17.
  3. Zhang, J. et al. (2021) Role of Human Mesangial-Tubular Crosstalk in Secretory IgA-Induced IgA Nephropathy.
    Kidney Blood Press Res. : Apr 161-12.

ELISA

RRID
AB_605423
UniProt
P01876
P01877
Entrez Gene
IGHA1
IGHA2
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
GO:0005624 membrane fraction

PHP133

149900 160372 160679

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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