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G-CSF

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Sandwich ELISA analysis of Human G-CSG using Rat anti human G-CSF (1012701) as a capture reagent and biotinylated Rat anti Human G-CSF (1012808) as a detection reagent with recombinant human G-CSF (PHP082B) as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Human serum (orange) and plasma (green) samples are tested undiluted.

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Recombinant Human G-CSF

Product Type
Recombinant Protein
Specificity
G-CSF

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Product Code Applications Pack Size List Price Your Price Qty
PHP082B
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Recombinant Human G-CSF is purified E.coli expressed recombinant human granulocyte colony stimulating factor (G-CSF). Granulocyte CSF is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and differentiates certain myeloid leukemic cells. Human and Murine G-CSF share approximately 75% amino acid sequence homology and have biological cross-reactivity.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant protein expressed in E.Coli
Buffer Solution
Lyophilised from 5mM Sodium Citrate pH4.0
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
1 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
18.7kDa (174 amino acid residues)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
P09919
Entrez Gene
CSF3
GO Terms
GO:0005125 cytokine activity
GO:0005130 granulocyte colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0019899 enzyme binding
GO:0019221 cytokine-mediated signaling pathway
GO:0030851 granulocyte differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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