G-CSF

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Recombinant Human G-CSF

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP082B Datasheet	FN SDS	10 µg	Log in		Get Quote

Summary
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Recombinant Human G-CSF is purified E.coli expressed recombinant human granulocyte colony stimulating factor (G-CSF). Granulocyte CSF is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and differentiates certain myeloid leukemic cells. Human and Murine G-CSF share approximately 75% amino acid sequence homology and have biological cross-reactivity.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant protein expressed in E.Coli
Buffer Solution: Lyophilised from 5mM Sodium Citrate pH4.0
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: 1 x 10⁷ units/mg
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 18.7kDa (174 amino acid residues)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at -20^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P09919
Entrez Gene: CSF3
GO Terms: GO:0005125 cytokine activity; GO:0005130 granulocyte colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0008284 positive regulation of cell proliferation; GO:0019899 enzyme binding; GO:0019221 cytokine-mediated signaling pathway; GO:0030851 granulocyte differentiation
Regulatory: For research purposes only

Applications of G-CSF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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