Defensin Beta 3

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Recombinant Human Defensin Beta 3

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP211 E FN WB 20 µg
Defensin beta 3 (BD-3) is a cationic non-haemolytic antimicrobial peptide and member of the beta-defensin family, highly expressed by skin and tonsils, which plays an important role in the innate immune response to potentially pathogenic microbes, including multiresistant S. aureus and vancomycin-resistant E. faecium.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 20ul 10mM acetic acid.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage, further dilutions may be made in buffer containing 0.1% bovine serum albumin (BSA).
Preparation
Purified recombinant defensin beta 3 expressed in E.coli
Preservative Stabilisers
None present
Activity
Exhibits chemotactic activity on monocytes.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Molecular Weight
5.1kD (45 amino acid residues)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P81534 Related reagents
Entrez Gene
DEFB103B Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0006965 positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
Regulatory
For research purposes only

Applications of Defensin Beta 3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP211 may be used in ELISA applications with either AHP1802 or AHP1802B.
Western Blotting
PHP211 may be used under reducing or non-reducing western blotting conditions with either AHP1802 or AHP1802B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Defensin Beta 3 AHP1802 0.1 mg E WB

Product Specific References

References for Defensin Beta 3

  1. Boesch, A.W. et al. (2013) A Multiplexed Assay to Detect Antimicrobial Peptides in Biological Fluids and Cell Secretions.
    J Immunol Methods. pii: S0022-1759(13)00241-X.