CCL24

100% Secure


Recombinant Human CCL24

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP296
Datasheet
E
SDS
10 µg loader

Recombinant Human CCL24

CCL24 is also known as C-C motif chemokine 24, Eotaxin-2, CK-beta-6, and MPIF-2. CCL24 is a chemokine which is induced in macophages and promotes recruitment and activation of immune cells including eosinophils, basophils, and Th2 cells by interacting with the CCR3 receptor. Therefore, CCL24 expression increases during inflammatory disease and allergic reaction, and CCL24 has been shown to perpetuate allergic reaction (Cho et al. 2016). CCL24 is involved with proinflammatory and profibrotic reactions, particularly fibrosis of the skin and lungs (Mor et al. 2019). Furthermore, CCL24 plays a role in intrahepatic fibrosis and liver damage (Segal-Salto et al. 2020). Elevated CCL24 has been associated with primary colorectal and liver metastatic tumors (Cheng et al. 2017).

This recombinant Human CCL24 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human CCL24 antibody, clone C05-2B5 (MCA6182GA) as a capture antibody and biotinylated Mouse anti Human CCL24 antibody, clone B01-5E2 (MCA6183B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by ion exchange chromatography
Buffer Solution
50 mM Tris-HCl, pH 8.0, 0.15 M Sodium Chloride
Preservative Stabilisers
1.0% Trehalose
Purity
≥90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Approx. Protein Concentrations
100 μg/ml after reconstitution
Protein Molecular Weight
10.5 kDa

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
O00175
Entrez Gene
CCL24
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0030335 positive regulation of cell migration
GO:0030838 positive regulation of actin filament polymerization
GO:0032855 positive regulation of Rac GTPase activity
Regulatory
For research purposes only

Applications of CCL24

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CCL24 MCA6182GA E 0.1 mg loader
Mouse anti Human CCL24:Biotin MCA6183B E 0.1 mg loader

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra