Serum Amyloid A

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Recombinant Horse Serum Amyloid A

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PEP006 E 0.1 mg
Recombinant Horse serum amyloid A (SAA) is a 120 aa recombinant protein produced in a eukaryotic expression system, which includes a proprietary 10 aa tag at the N-terminus.

Serum amyloid A proteins are a family of acute phase proteins, produced primarily by the liver, highly conserved in vertebrate species, and used as markers of inflammation.

Equine SAA, as with serum amyloid A in other species, is present in different isoforms, this recombinant protein corresponds to isoform 1 (SAA1). Equine SAA is composed of 110 amino acids and differs from human SAA by having an 8 aa sequence insert in the central region of the sequence.

With similar biochemical properties to the native equine SAA, this reagent may be used as a standard in immunoassays for measurement of levels of horse serum amyloid A.

Product Details

Target Species
Horse
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified by affinity and reversed-phase chromatography
Buffer Solution
0.01M HCl
Preservative Stabilisers
None present
Purity
>95%
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Molecular Weight
13.6 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P19857 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0034364 high-density lipoprotein particle
Regulatory
For research purposes only

Applications of Serum Amyloid A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.