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IL-5

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Bovine Interleukin-5 ELISA

Recombinant Bovine Interleukin-5 (PBP041) detected using Rabbit anti Bovine Interleukin-5 (AHP2818) as the capture reagent and Rabbit anti Bovine Interleukin-5:Biotin (AHP2818B) as the detection reagent followed by Streptavidin:HRP.

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Recombinant Bovine Interleukin-5

Product Type
Recombinant Protein
Specificity
IL-5

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Product Code Applications Pack Size List Price Your Price Qty
PBP041
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SDS Safety Datasheet SDS
E WB 5 µg loader
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Recombinant Bovine Interleukin-5 (IL-5) is a recombinant protein corresponding to aa 22-134 of native bovine IL-5.

IL-5 is a regulatory cytokine that induces terminal differentiation of late-developing B cells to immunoglobulin secreting cells.

There is >85% identity with feline, bovine, ovine, canine, and human IL-5 sequences at the nucleotide and protein level.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
13.0 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2818 as a coating antibody and AHP2818B as a detection antibody.

UniProt
P52173
Entrez Gene
IL5
GO Terms
GO:0005125 cytokine activity
GO:0005137 interleukin-5 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
View more products with IL-5 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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