IL-5
Recombinant Bovine Interleukin-5 (IL-5) is a recombinant protein corresponding to aa 22-134 of native bovine IL-5.
IL-5 is a regulatory cytokine that induces terminal differentiation of late-developing B cells to immunoglobulin secreting cells.
There is >85% identity with feline, bovine, ovine, canine, and human IL-5 sequences at the nucleotide and protein level.
IL-5 is a regulatory cytokine that induces terminal differentiation of late-developing B cells to immunoglobulin secreting cells.
There is >85% identity with feline, bovine, ovine, canine, and human IL-5 sequences at the nucleotide and protein level.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 13.0 kDa
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P52173
- Entrez Gene
- IL5
- GO Terms
- GO:0005125 cytokine activity
- GO:0005137 interleukin-5 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of IL-5
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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