IL-1 Beta
Recombinant Bovine Interleukin-1 beta used to create the standard curve for the measurement of IL-β in mik during experimental S. epidermidisand S.simulans infection by ELISA.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-○-) and S. simulans (-●-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.
From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
Recombinant Bovine Interleukin-1 beta used to create the standard curve for the measurement of IL-β in mik during experimental S. epidermidisand S.simulans infection by ELISA.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-□-) and spontaneously eliminated (-■-) S. epidermidis and S. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.
From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
Recombinant Bovine Interleukin-1 beta is a recombinant peptide corresponding to full-length mature Bovine Interleukin-1 beta (IL-1 beta) expressed by a number of cell types including monocytes, macrophages and endothelial cells.
Interleukin-1 beta, a pro-inflammatory cytokine has a wide range of effects, such as promoting T and B cell proliferation and inducing neutrophil chemotaxis.
Interleukin-1 beta, a pro-inflammatory cytokine has a wide range of effects, such as promoting T and B cell proliferation and inducing neutrophil chemotaxis.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Recombinant Protein - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water.
Further dilutions should be made in a buffer containing carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Purity
- >95% by SDS PAGE analysis
- Approx. Protein Concentrations
- 40ug/ml after reconstitution
- Protein Molecular Weight
- 17.7kD
- Endotoxin Level
- <0.5EU/ug
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P09428 Related reagents
- Entrez Gene
- IL1B Related reagents
- GO Terms
- GO:0001660 fever generation
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0051781 positive regulation of cell division
- Regulatory
- For research purposes only
Applications of IL-1 Beta
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IL-1 Beta
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Stradner, M.H. et al. (2008) Spingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytes.
Osteoarthritis Cartilage. 16: 305-11. -
Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
Clin Vaccine Immunol. 17(11):1797-809. -
Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 42: 49. -
Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.
Trop Anim Health Prod. 44: 651-5. -
Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. pii: S0165-2427(15)00195-6. -
Carroll JA et al. (2016) In utero exposure to LPS alters the postnatal acute-phase response in beef heifers.
Innate Immun. Nov 21. pii: 1753425916678472. [Epub ahead of print] -
Doull L et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. 168 (1-2): 97-102.
