Find out more about A.I.den - our SearchBot

IL-1 Beta

ISO 9001 Quality Assurance Manufacturing | Print
Recombinant Bovine Interleukin-1 beta thumbnail image 1 Recombinant Bovine Interleukin-1 beta thumbnail image 2
Recombinant Bovine Interleukin-1 beta gallery image 1
Published customer image:
Recombinant Bovine Interleukin-1 beta used to create the standard curve for the measurement of IL-β in mik during experimental S. epidermidisand S.simulans infection by ELISA.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-⃝-) and S. simulans (-●-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Recombinant Bovine Interleukin-1 beta gallery image 2
Published customer image:
Recombinant Bovine Interleukin-1 beta used to create the standard curve for the measurement of IL-β in mik during experimental S. epidermidisand S.simulans infection by ELISA.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-□-) and spontaneously eliminated (-■-) S. epidermidis and S. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Filter by Application:

E

Recombinant Bovine Interleukin-1 beta

Product Type
Recombinant Protein
Specificity
IL-1 Beta

Quick Links:

Antibody Quality and Performance Guarantee Antibody Quality and Performance GuaranteeAntibody Quality and
Performance Guarantee
Unrivaled technical support Unrivalled technical supportUnrivaled
Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
PBP008
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN 20 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Recombinant Bovine Interleukin-1 beta is a recombinant peptide corresponding to full-length mature Bovine Interleukin-1 beta (IL-1 beta) expressed by a number of cell types including monocytes, macrophages and endothelial cells.

Interleukin-1 beta, a pro-inflammatory cytokine has a wide range of effects, such as promoting T and B cell proliferation and inducing neutrophil chemotaxis.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Recombinant Protein - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water.

Further dilutions should be made in a buffer containing carrier protein.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Approx. Protein Concentrations
40ug/ml after reconstitution
Protein Molecular Weight
17.7kD
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
ELISA
This product may be used as a standard for ELISA applications with the following interleukin-1 beta antibodies: AHP851Z, AHP851B or MCA1658.

References for IL-1 Beta

  1. Stradner, M.H. et al. (2008) Spingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytes.
    Osteoarthritis Cartilage. 16: 305-11.
  2. Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
    Clin Vaccine Immunol. 17(11):1797-809.
  3. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  4. Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.
    Trop Anim Health Prod. 44: 651-5.
  5. L, D. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  6. JA, C. et al. (2017) In utero exposure to LPS alters the postnatal acute-phase response in beef heifers.
    Innate Immun. 23 (1): 97-108.

ELISA

UniProt
P09428
Entrez Gene
IL1B
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division
View more products with IL-1 BETA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Bovine Products