IL-1 Beta

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Recombinant Bovine Interleukin-1 beta

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP008 E FN 20 µg
Recombinant Bovine Interleukin-1 beta is a recombinant peptide corresponding to full-length mature Bovine Interleukin-1 beta (IL-1 beta) expressed by a number of cell types including monocytes, macrophages and endothelial cells.

Interleukin-1 beta, a pro-inflammatory cytokine has a wide range of effects, such as promoting T and B cell proliferation and inducing neutrophil chemotaxis.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Recombinant Protein - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water.

Further dilutions should be made in a buffer containing carrier protein.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Approx. Protein Concentrations
40ug/ml after reconstitution
Protein Molecular Weight
17.7kD
Endotoxin Level
<0.5EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P09428 Related reagents
Entrez Gene
IL1B Related reagents
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of IL-1 Beta

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
ELISA
This product may be used as a standard for ELISA applications with the following interleukin-1 beta antibodies: AHP851Z, AHP851B or MCA1658.

Application Based External Images

ELISA

Product Specific References

References for IL-1 Beta

  1. Stradner, M.H. et al. (2008) Spingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytes.
    Osteoarthritis Cartilage. 16: 305-11.
  2. Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
    Clin Vaccine Immunol. 17(11):1797-809.
  3. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  4. Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.
    Trop Anim Health Prod. 44: 651-5.
  5. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. pii: S0165-2427(15)00195-6.
  6. Carroll JA et al. (2016) In utero exposure to LPS alters the postnatal acute-phase response in beef heifers.
    Innate Immun. Nov 21. pii: 1753425916678472. [Epub ahead of print]
  7. Doull L et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.