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IL-1 Alpha

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Bovine Interleukin-1 alpha ELISA

Recombinant Bovine interleukin-1 alpha(PBP031) detected using Rabbit anti Bovine interleukin-1 alpha (AHP2373) as the capture reagent and Rabbit anti Bovine interleukin-1 alpha:Biotin (AHP2373B) as the detection reagent followed by Streptavidin:HRP

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Recombinant Bovine Interleukin-1 Alpha

Product Type
Recombinant Protein
Specificity
IL-1 Alpha

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
PBP031
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN 5 µg loader
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Search for Batch Specific Datasheets

Recombinant Bovine interleukin-1 alpha (IL-1α) is a recombinant protein corresponding to aa 120-268 of native bovine IL-1α.

Interleukin-1α is a pleiotropic pro-inflammatory cytokine and member of the IL-1 superfamily. It is secreted by activated macrophages and monocytes and is involved in the modulation of a wide range of immune and inflammatory responses.

IL-1α and the closely related IL-1β, are both involved in haematopoiesis and exhibit many identical immunomodulatory properties, binding to the same type I interleukin-1 receptor, also known as CD121a.

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
17.1 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2373 as a coating antibody and AHP2373B as a detection antibody.

UniProt
P08831
Entrez Gene
IL1A
GO Terms
GO:0001660 fever generation
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005507 copper ion binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0034605 cellular response to heat
GO:0046688 response to copper ion
GO:0051781 positive regulation of cell division
View more products with IL-1 ALPHA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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